伊朗非酒精性脂肪性肝病患者的血清对氧磷酶和芳基酯酶活性

Serum paraoxonase and arylesterase activities in Iranian patients with nonalcoholic fatty liver disease.

作者信息

Hashemi Mohammad, Bahari Ali, Hashemzehi Norallah, Moazeni-Roodi Abdolkarim, Shafieipour Sara, Bakhshipour Alireza, Ghavami Saeid

机构信息

Department of Clinical Biochemistry, School of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran; Cellular and Molecular Biology Research Center, Zahedan University of Medical Sciences, Zahedan, Iran.

出版信息

Pathophysiology. 2012 Apr;19(2):115-9. doi: 10.1016/j.pathophys.2012.04.001. Epub 2012 May 1.

DOI:10.1016/j.pathophys.2012.04.001

PMID:22555050

Abstract

BACKGROUND

The aim of the study was to investigate paraoxonase-1 (PON1) and aryl esterase (ARE) activities in patients with nonalcoholic fatty liver disease (NAFLD).

MATERIALS AND METHODS

This case-control study was done on 83 subjects with confirmed NAFLD (50 male, 33 female, age; 40.46±12.13 years) and 138 healthy individuals (75 male, 63 female; age; 40.94±14.50 years). PON1, salt-stimulated PON1 and ARE activities were determined using paraoxon and phenyl acetate as substrate, respectively.

RESULTS

The levels of PON1 activities in NAFLD and healthy individuals were 90.83±63.65 IU/L and 79.41±68.14 IU/L, respectively. There was no significant differences regarding PON1 activity between NAFLD and healthy subjects (p=0.229). While, ARE activity was significantly higher in NAFLD (83.34±28.36 KU/L) than in normal subjects (64.06±27.49 KU/L) (p<0.001).

CONCLUSIONS

Our results showed that PON1 activity is not a promising biomarker for the evaluation of NAFLD while arylesterase may have, but further studies in larger samples with different ethnic groups are required to validate our findings.

摘要

背景

本研究旨在调查非酒精性脂肪性肝病（NAFLD）患者的对氧磷酶-1（PON1）和芳基酯酶（ARE）活性。

材料与方法

本病例对照研究对83例确诊为NAFLD的受试者（50例男性，33例女性，年龄40.46±12.13岁）和138名健康个体（75例男性，63例女性；年龄40.94±14.50岁）进行。分别以对氧磷和苯乙酸为底物测定PON1、盐刺激的PON1和ARE活性。

结果

NAFLD患者和健康个体的PON1活性水平分别为90.83±63.65 IU/L和79.41±68.14 IU/L。NAFLD患者与健康受试者之间的PON1活性无显著差异（p = 0.229）。然而，NAFLD患者的ARE活性（83.34±28.36 KU/L）显著高于正常受试者（64.06±27.49 KU/L）（p<0.001）。