Sarioglu Nurhan, Hismiogullari Adnan Adil, Erel Fuat, Demir Dudu, Gencer Nahit
Department of Pulmonary Diseases, Faculty of Medicine, Balikesir University Balikesir, Turkey.
Department of Biochemistry, Faculty of Medicine, Balikesir University, Balikesir, Turkey.
Iran J Allergy Asthma Immunol. 2015 Feb;14(1):60-6.
Oxidative stress is involved in the pathogenesis of asthma. Paraoxonase 1 (PON1) and arylesterase are esterase enzymes displaying antioxidant characteristics. PON1 activity varies widely among individuals and ethnic groups, partly related to polymorphisms. The aim of this study was to determine the activities of PON1 and arylesterase including the phenotype distribution of PON1 in asthmatic patients and healthy subjects. Forty-nine asthmatic patients and 41 healthy people were included in this study. Serum PON1 and arylesterase activities were determined by spectrophotometric assays, as well as the lipid profiles. The PON1 ratio (salt stimulated paraoxonase/arylesterase) was trimodally distributed and this ratio was used to determine the individual phenotypes of all subjects. The PON1 activity in the asthmatic patients was significantly lower (p=0.024) when compared to the healthy control group, however no significant difference in the activity of arylesterase was observed between the two groups. The prevalence of the PON1 phenotypes in the asthmatic population were 26.5%, 16.3% and 57.2 % for QQ, QR and RR, respectively. PON1 activity was significantly lower in asthmatic patients; in addition, the results of this investigation indicated that PON1 RR phenotype may be an important risk factor in asthma disease.
氧化应激参与哮喘的发病机制。对氧磷酶1（PON1）和芳基酯酶是具有抗氧化特性的酯酶。PON1活性在个体和种族群体中差异很大，部分与基因多态性有关。本研究的目的是测定哮喘患者和健康受试者中PON1和芳基酯酶的活性，包括PON1的表型分布。本研究纳入了49例哮喘患者和41名健康人。通过分光光度法测定血清PON1和芳基酯酶活性以及血脂谱。PON1比率（盐刺激对氧磷酶/芳基酯酶）呈三峰分布，该比率用于确定所有受试者的个体表型。与健康对照组相比，哮喘患者的PON1活性显著降低（p = 0.024），然而两组之间芳基酯酶活性未观察到显著差异。哮喘人群中PON1表型的患病率分别为QQ型26.5%、QR型16.3%和RR型57.2%。哮喘患者的PON1活性显著降低；此外，本研究结果表明PON1 RR表型可能是哮喘疾病的一个重要危险因素。
