针对表达胸苷激酶的单纯疱疹病毒的选择性检测法。
Selective assay for herpes simplex viruses expressing thymidine kinase.
作者信息
Campione-Piccardo J, Rawls W E, Bacchetti S
出版信息
J Virol. 1979 Aug;31(2):281-7. doi: 10.1128/JVI.31.2.281-287.1979.
Abstract
A technique for selecting herpes simplex viruses expressing the viral thymidine kinase (TK+) from a population of predominantly TK- viruses was developed. This was accomplished by infecting TK- cells and incubating the cultures under a liquid overlay medium containing methotrexate. Since the TK- cells survive in this medium for only a limited period of time, it was necessary to add fresh uninfected TK- cells 48 h after infection. The technique allowed the detection and quantitation of the TK+ virus fraction in mixtures of TK+ and TK- viruses where the TK+ fraction was present in frequencies as low as 10(-5). It was also used to estimate reversion frequencies and to obtain and analyze TK+ revertants from TK- mutant strains of herpes simplex virus type 1.
摘要
开发了一种从主要为胸苷激酶阴性（TK-）的病毒群体中选择表达病毒胸苷激酶（TK+）的单纯疱疹病毒的技术。这是通过感染TK-细胞并在含有甲氨蝶呤的液体覆盖培养基下培养来实现的。由于TK-细胞在这种培养基中仅能存活有限的时间，因此有必要在感染后48小时添加新鲜的未感染TK-细胞。该技术能够检测和定量TK+和TK-病毒混合物中TK+病毒组分，其中TK+组分的频率低至10^(-5)。它还用于估计回复频率，并从1型单纯疱疹病毒的TK-突变株中获得并分析TK+回复株。
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本文引用的文献
1
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Virology. 1964 Apr;22:493-502. doi: 10.1016/0042-6822(64)90070-4.
2
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Proc Soc Exp Biol Med. 1964 Mar;115:814-6. doi: 10.3181/00379727-115-29045.
3
4
Induction of both thymidine and deoxycytidine kinase activity by herpes viruses.疱疹病毒对胸苷激酶和脱氧胞苷激酶活性的诱导作用。
J Gen Virol. 1974 Sep;24(3):465-80. doi: 10.1099/0022-1317-24-3-465.
5
Isolation, complementation and preliminary phenotypic characterization of temperature-sensitive mutants of herpes simplex virus type 2.2型单纯疱疹病毒温度敏感突变体的分离、互补及初步表型特征分析
Virology. 1974 Feb;57(2):554-65. doi: 10.1016/0042-6822(74)90194-9.
6
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J Virol. 1974 Jan;13(1):140-5. doi: 10.1128/JVI.13.1.140-145.1974.
7
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8
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Mol Cell Biochem. 1976 Jun 15;11(3):161-82. doi: 10.1007/BF01744997.
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