马来半岛经济欠发达社区人群钩虫感染的分子鉴定。
Molecular identification of human hookworm infections in economically disadvantaged communities in Peninsular Malaysia.
机构信息
Department of Parasitology, Faculty of Medicine, University of Malaya, Kuala Lumpur Malaysia.
出版信息
Am J Trop Med Hyg. 2012 May;86(5):837-42. doi: 10.4269/ajtmh.2012.11-0446.
Abstract
Species identification of human hookworm infections among eight communities in rural areas of Peninsular Malaysia was determined during 2009-2011. Fecal samples were examined by microscopy and subsequently, the internal transcribed spacer 2 and 28S ribosomal RNA region of Necator americanus and Ancylostoma spp. were sequenced. Overall, 9.1% (58 of 634) were identified positive by microscopy for hookworm infection, and 47 (81.0%) of 58 were successfully amplified and sequenced. Sequence comparison found that N. americanus (87.2%) was the most predominant hookworm identified, followed by Ancylostoma ceylanicum (23.4%). No A. duodenale infection was detected in this study. Detection of A. ceylanicum in humans highlighted the zoonotic transmission among humans living near dogs. Thus, implementation of effective control measures for hookworm infections in future should seriously consider this zoonotic implication.
摘要
2009-2011 年，在马来西亚半岛 8 个农村社区对人类钩虫感染进行了物种鉴定。通过显微镜检查粪便样本，随后对美洲板口线虫和Ancylostoma spp.的内部转录间隔区 2 和 28S 核糖体 RNA 区域进行了测序。总体而言，通过显微镜检查发现 9.1%（634 例中的 58 例）钩虫感染呈阳性，其中 58 例中有 47 例（81.0%）成功扩增和测序。序列比较发现，美洲板口线虫（87.2%）是鉴定出的最主要钩虫，其次是锡兰钩口线虫（23.4%）。本研究未检测到十二指肠钩虫感染。在人类中检测到锡兰钩口线虫表明，生活在狗附近的人类之间存在动物源性传播。因此，未来实施钩虫感染的有效控制措施应认真考虑这种动物源性影响。
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