Universidade do Estado de Minas Gerais, Avenida Juca Stockler, 1130, CEP 37900-106, Nossa Sra. das Gracas, Passos, Minas Gerais, Brazil.
Universidade Federal de Minas Gerais, Instituto de Ciências Biológicas, Avenida Presidente Antônio Carlos, 6627, Departamento de Parasitologia, L4 237, Laboratório de Parasitologia Molecular, Pampulha, CEP 31270-901, Belo Horizonte, Minas Gerais, Brazil.
Sci Rep. 2020 Feb 20;10(1):3006. doi: 10.1038/s41598-020-59874-8.
Hookworms are intestinal parasites that cause major public health problems, especially in developing countries. To differentiate eggs from different hookworm species, it is necessary to use molecular methodologies, since the eggs are morphologically similar. Here, we performed the molecular identification of single hookworm eggs from six Brazilian states. Of the 634 eggs individually analyzed, 98.1% (622/634) represented Necator americanus, and surprisingly, 1.9% (12/634 eggs from the same patient) represented Ancylostoma caninum. DNA analysis of the A. caninum-positive stool sample revealed no contamination with animal feces. This is the first report of the presence of A. caninum eggs in human feces, which may have a direct implication for the epidemiology of hookworm infection caused by this species. This suggests the need for special attention regarding prophylaxis, as different reservoirs, previously not described, may have great relevance for the spread of A. caninum.
钩虫是肠道寄生虫，会引起重大公共卫生问题，尤其是在发展中国家。为了区分不同钩虫物种的虫卵，有必要使用分子方法，因为这些虫卵在形态上相似。在这里，我们对来自巴西六个州的单个钩虫卵进行了分子鉴定。在 634 个单独分析的虫卵中，98.1%（622/634）代表美洲板口线虫，令人惊讶的是，1.9%（来自同一患者的 12/634 个虫卵）代表犬钩口线虫。对犬钩口线虫阳性粪便样本的 DNA 分析显示没有动物粪便污染。这是首次在人类粪便中发现犬钩口线虫虫卵，这可能直接影响该物种引起的钩虫感染的流行病学。这表明需要特别注意预防措施，因为以前未描述的不同储存宿主可能与犬钩口线虫的传播有很大关系。
