Garate T, Conraths F J, Harnett W, Büttner D W, Parkhouse R M
Division of Immunology, National Institute for Medical Research, Mill Hill, London, U.K.
Trop Med Parasitol. 1990 Sep;41(3):245-50.
Specific, serological diagnosis is one of the main goals in onchocerciasis research. To date this objective has been hampered by (a) scarcity of parasite material, and (b) antigenic cross-reaction between Onchocerca volvulus and other nematode species. In order to obtain specific antigens, and in amounts suitable for study, molecular biological techniques have been adopted. A lambda gt11 cDNA expression library prepared from O. volvulus adult female worms was screened using infected human sera from onchocerciasis patients and rabbit hyperimmune sera raised against Onchocerca and genus-specific Onchocerca antigen extracts. Five clones were selected and their inserts expressed as beta-galactosidase fusion proteins. The fusion proteins were examined using individual sera from patients with O. volvulus or Wuchereria bancrofti infections. Three of the fusion proteins were recognised by more than 80% of O. volvulus sera and exhibited weak reactivity with a few W. bancrofti sera. One of these three clones was recognised to a significantly greater degree by sera from sowda than from generalised onchocerciasis patients.
特异性血清学诊断是盘尾丝虫病研究的主要目标之一。迄今为止，这一目标受到以下因素的阻碍：（a）寄生虫材料稀缺，以及（b）盘尾丝虫与其他线虫物种之间的抗原交叉反应。为了获得适合研究的特定抗原，已采用分子生物学技术。使用来自盘尾丝虫病患者的感染人血清以及针对盘尾丝虫属特异性抗原提取物产生的兔超免疫血清，筛选了从盘尾丝虫成年雌性虫体制备的λgt11 cDNA表达文库。选择了5个克隆，并将其插入片段表达为β-半乳糖苷酶融合蛋白。使用来自盘尾丝虫或班氏吴策线虫感染患者的个体血清检测融合蛋白。超过80%的盘尾丝虫血清识别出其中三种融合蛋白，并且与少数班氏吴策线虫血清表现出微弱反应性。这三个克隆中的一个被来自重度盘尾丝虫性皮炎患者的血清识别的程度明显高于来自全身性盘尾丝虫病患者的血清。
