Cabrera Z, Buttner D W, Parkhouse R M
National Institute for Medical Research Mill Hill, London, UK.
Clin Exp Immunol. 1988 Nov;74(2):223-9.
Individual human Ig class responses to Onchocerca volvulus antigens have been evaluated by Western blotting using sera from cases of generalized onchocerciasis and chronic hyper-reactive onchocerciasis (Sowda). in all cases except IgG3 the patterns of recognition by human antibody classes were similar in Sowda and generalized onchocerciasis. Weak or undetectable responses were seen with IgG1, IgG2 and IgM. The total profiles of antigens recognized by the other Ig classes were different, although in some cases certain bands were commonly identified. The result with IgG3, however, was striking. Here, two major antigens (9 kD and 72kD) were recognized by IgG3 antibodies in Sowda sera but not generalized onchocerciasis sera. Furthermore, these two antigens were not recognised by any other Ig class, either in generalized or Sowda onchocerciasis, nor were they detected by antibodies of any class present in a collection of sera representative of other nematode infections. This difference in the IgG3 response was so pronounced that Sowda sera could be distinguished from generalized onchocerciasis sera by an IgG3-specific ELISA assay with a PBS parasite extract as the antigen. Thus, a correlation has been established between one particular clinical condition of onchocerciasis (Sowda) and a serological response, defined in terms of both the parasite antigens and an immunoglobulin class restricted antibody response.
通过蛋白质印迹法，利用来自全身性盘尾丝虫病和慢性超敏反应性盘尾丝虫病（Sowda）病例的血清，评估了个体人类免疫球蛋白（Ig）类对盘尾丝虫抗原的反应。除IgG3外，在Sowda和全身性盘尾丝虫病中，人类抗体类别的识别模式相似。IgG1、IgG2和IgM的反应较弱或无法检测到。尽管在某些情况下某些条带被共同识别，但其他Ig类识别的抗原总谱不同。然而，IgG3的结果很显著。在Sowda血清中，IgG3抗体识别两种主要抗原（9kD和72kD），而在全身性盘尾丝虫病血清中则不识别。此外，在全身性或Sowda盘尾丝虫病中，这两种抗原都未被任何其他Ig类识别，在一组代表其他线虫感染的血清中存在的任何类别的抗体也未检测到它们。IgG3反应的这种差异非常明显，以至于以PBS寄生虫提取物为抗原的IgG3特异性ELISA检测可以区分Sowda血清和全身性盘尾丝虫病血清。因此，在盘尾丝虫病的一种特定临床状况（Sowda）与一种血清学反应之间建立了相关性，这种血清学反应是根据寄生虫抗原和免疫球蛋白类限制性抗体反应来定义的。
