Dengue Group, National Institute of Virology, 20-A, Ambedkar road, Pune, Maharashtra, India.
Virol J. 2012 May 4;9:86. doi: 10.1186/1743-422X-9-86.
Altered plasma concentrations of vitamin D and mannose binding lectin (MBL), components of innate immunity, have been shown to be associated with the pathogenesis of viral infections. The objective of the present study was to find out whether plasma concentrations of MBL and vitamin D are different in patients with dengue fever (DF) and dengue hemorrhagic fever (DHF).
The plasma concentrations of vitamin D and MBL were assessed in 48 DF cases, 45 DHF cases and 20 apparently healthy controls using ELISA based methods. Vitamin D concentrations were found to be higher among both DF and DHF cases as compared to healthy controls (P < 0.005 and P < 0.001). Vitamin D concentrations were not different between DF and DHF cases. When the dengue cases were classified into primary and secondary infections, secondary DHF cases had significantly higher concentrations of vitamin D as compared to secondary DF cases (P < 0.050). MBL concentrations were not significantly different between healthy controls and dengue cases. MBL concentrations were observed to be lower in DHF cases as compared to DF cases (P < 0.050). Although MBL levels were not different DF and DHF cases based on immune status, the percentage of primary DHF cases (50%) having MBL levels lower than 500 ng/ml were less compared to primary DF cases (P = 0.038).
The present study suggests that higher concentrations of vitamin D might be associated with secondary DHF while deficiency of MBL may be associated with primary DHF.
已证实，改变的血浆维生素 D 和甘露糖结合凝集素（MBL）浓度，先天免疫的组成部分，与病毒感染的发病机制相关。本研究的目的是查明登革热（DF）和登革出血热（DHF）患者的 MBL 和维生素 D 血浆浓度是否存在差异。
采用 ELISA 法检测了 48 例 DF 病例、45 例 DHF 病例和 20 名健康对照者的 MBL 和维生素 D 血浆浓度。与健康对照组相比，DF 和 DHF 病例的维生素 D 浓度均较高（P<0.005 和 P<0.001）。DF 和 DHF 病例之间维生素 D 浓度无差异。当将登革热病例分为原发性和继发性感染时，与继发性 DF 病例相比，继发性 DHF 病例的维生素 D 浓度显著较高（P<0.050）。健康对照组和登革热病例之间 MBL 浓度无差异。与 DF 病例相比，DHF 病例的 MBL 浓度较低（P<0.050）。尽管根据免疫状态，DF 和 DHF 病例之间的 MBL 水平无差异，但原发性 DHF 病例（50%）MBL 水平低于 500ng/ml 的比例低于原发性 DF 病例（P=0.038）。
本研究表明，较高的维生素 D 浓度可能与继发性 DHF 相关，而 MBL 缺乏可能与原发性 DHF 相关。
