Fabris Victoria T, Lodillinsky Catalina, Pampena María Betina, Belgorosky Denise, Lanari Claudia, Eiján Ana María
Laboratory of Hormonal Carcinogenesis, Instituto de Biología y Medicina Experimental (Consejo Nacional de Investigaciones Científicas y Técnicas-CONICET), Buenos Aires, Argentina.
Cancer Genet. 2012 Apr;205(4):168-76. doi: 10.1016/j.cancergen.2012.02.002.
Bladder cancer is frequently associated with chromosomal abnormalities, and the complexity of karyotypes increases with tumor progression. The murine model MB49 is one of the most widely studied models of bladder cancer. We developed the invasive cell line MB49-I by successive in vivo passages of MB49 primary tumors. Because little is known about the chromosomal alterations of this model, our goal was to perform cytogenetic analyses of the MB49 and MB49-I lines. The karyotypes of both lines were analyzed by G-banding and fluorescence in situ hybridization techniques. Both lines were composed of two cell subpopulations, a diploid population, which was found mainly in the MB49 line, and the tetraploid population, which was found mainly in the MB49-I line. A translocation between chromosomes 5 and 9 and an isochromosome of chromosome 19 were observed in the subpopulations of both lines. New structural abnormalities and additional chromosomal imbalances were detected in the MB49-I line. Tumor progression in the MB49/MB49-I model was associated with a selection of polyploid cells with accompanying chromosomal abnormalities. This model may be advantageous for the study of the genetic changes associated with the progression of bladder cancer.
膀胱癌常与染色体异常相关，并且核型的复杂性随肿瘤进展而增加。小鼠模型MB49是研究最广泛的膀胱癌模型之一。我们通过MB49原发性肿瘤的连续体内传代培养出侵袭性细胞系MB49-I。由于对该模型的染色体改变了解甚少，我们的目标是对MB49和MB49-I细胞系进行细胞遗传学分析。通过G显带和荧光原位杂交技术分析了两个细胞系的核型。两个细胞系均由两个细胞亚群组成，一个是二倍体细胞群，主要存在于MB49细胞系中，另一个是四倍体细胞群，主要存在于MB49-I细胞系中。在两个细胞系的亚群中均观察到5号和9号染色体之间的易位以及19号染色体的等臂染色体。在MB49-I细胞系中检测到新的结构异常和额外的染色体失衡。MB49/MB49-I模型中的肿瘤进展与具有伴随染色体异常的多倍体细胞的选择有关。该模型可能有利于研究与膀胱癌进展相关的基因变化。
