Cowell J K
J Natl Cancer Inst. 1980 Nov;65(5):955-61.
Nine epithelial tumor cell lines that were derived from inbred C57BL/lcrf-at adult male mouse bladder and were transformed in vitro were cytogenetically analyzed. Most cell lines were near-diploid and showed only minor karyotypic deviations from normal. Tetraploid cell lines showed duplication of chromosome markers, which suggested that they arose originally as near-diploid cell lines with minor karyotypic changes. Chromosome abnormalities were consistent among different cell lines and involved chromosomes #6, #3, and #15. The Y-chromosome was frequently missing. Identical chromosome abnormalities were present in cell lines that arose in control, dimethyl sulfoxide-treated, or 7, 12-dimethylbenz[a]anthracene-treated cultures. The most frequent abnormality was was the presence of three copies (trisomy) of chromosome #6. Few structural chromosome abnormalities were detected, although a marker chromosome derived from chromosome #3 was present in several cell lines. Two cell lines had excesses of chromosome #15. These results suggest that excesses of individual chromosomes or parts of chromosomes, particularly chromosome #6, may be important in the expression of the malignant phenotype in mouse bladder epithelium.
对九条来源于近交系C57BL/lcrf-at成年雄性小鼠膀胱且在体外转化的上皮肿瘤细胞系进行了细胞遗传学分析。大多数细胞系接近二倍体，仅显示出与正常情况轻微的核型偏差。四倍体细胞系显示出染色体标记的重复，这表明它们最初是作为具有轻微核型变化的近二倍体细胞系出现的。不同细胞系之间的染色体异常是一致的，涉及6号、3号和15号染色体。Y染色体经常缺失。在对照、二甲基亚砜处理或7,12-二甲基苯并[a]蒽处理的培养物中产生的细胞系中存在相同的染色体异常。最常见的异常是6号染色体出现三个拷贝（三体）。虽然在几个细胞系中存在一条来源于3号染色体的标记染色体，但检测到的染色体结构异常很少。两个细胞系中15号染色体过多。这些结果表明，单个染色体或染色体部分的过多，特别是6号染色体，可能在小鼠膀胱上皮恶性表型的表达中起重要作用。
