Instituto Universitario de Investigación en Ciencias Ambientales, Dept. Producción Animal y Ciencia de los Alimentos, Universidad de Zaragoza, Spain.
Anaerobe. 2012 Jun;18(3):344-9. doi: 10.1016/j.anaerobe.2012.04.004. Epub 2012 Apr 24.
In order to study the microbial caecal ecosystem of wild and domestic rabbits through the fermentation characteristics and concentration and diversity of bacterial and archaeal communities, caecal samples from sixteen wild rabbits (WR) were contrasted with two groups (n = 4) of farm rabbits receiving low (LSF) or high (HSF) soluble fibre diets from 28 (weaning) to 51 days of age. DNA was extracted for quantifying bacteria and Archaea by qPCR and for biodiversity analysis of microbial communities by DGGE. Samples from WR had lower caecal pH and ammonia and higher volatile fatty acids concentration than farm animals. Lower acetate and higher butyrate proportions were detected in WR. Bacterial and archaeal DGGE profiles were clearly different between wild and farm rabbits, and diet-affected population of farm rabbits. Similarity index of bacteria was lower than 0.40 among WR, and 0.52 among farm rabbits. In conclusion, caecal fermentation characteristics differ between wild and farm rabbits, which harbour clearly different bacterial and archaeal communities. In farm rabbits, diversity is influenced by the dietary level of soluble fibre.
为了通过发酵特性以及细菌和古菌群落的浓度和多样性来研究野生和家兔的盲肠微生物生态系统，对比了 16 只野生兔（WR）和两组接受低（LSF）或高（HSF）可溶性纤维饮食的家兔（每组 4 只）的盲肠样本，研究从 28 天（断奶）到 51 天。通过 qPCR 定量细菌和古菌 DNA，并通过 DGGE 分析微生物群落的生物多样性。WR 的盲肠 pH、氨和挥发性脂肪酸浓度均低于农场动物，乙酸盐比例较低，丁酸盐比例较高。野生和农场兔子之间的细菌和古菌 DGGE 图谱明显不同，并且农场兔子的饮食也会影响菌群。WR 之间的细菌相似性指数低于 0.40，农场兔子的相似性指数低于 0.52。总之，野生和家兔的盲肠发酵特性不同，其携带的细菌和古菌群落也明显不同。在家兔中，饮食中可溶性纤维的水平会影响多样性。
