Mohammadzadeh Hamid, Yáñez-Ruiz David R, Martínez-Fernandez Gonzalo, Abecia Leticia
Animal Nutrition Institute, Estación Experimental del Zaidín, CSIC, Profesor Albareda 1, 18008 Granada, Spain; Department of Animal Science, Faculty of Agriculture, University of Tabriz, Iran.
Animal Nutrition Institute, Estación Experimental del Zaidín, CSIC, Profesor Albareda 1, 18008 Granada, Spain.
Anaerobe. 2014 Oct;29:52-8. doi: 10.1016/j.anaerobe.2013.11.012. Epub 2013 Dec 11.
The objective of this work was to compare the biomass and community structure of bacteria, protozoa and archaea communities in samples of rumen and faeces of goats and to what extent the diet (alfalfa hay with or without supplemented oats) offered to them exert an influence. Four cannulated adult goats fistulated in the rumen were used in a cross over design experiment in two experimental periods of 26 days, consisting in 14 days of adaptation, 7 days of sampling rumen contents and 5 days of digestibility measurement. Bacterial, protozoa and archaeal biomass and the communities' structure was assessed by real time PCR (qPCR) and denaturing gradient gel electrophoresis (DGGE), respectively. The numbers of archaea and bacteria in both rumen and faeces were higher and lower, respectively, in animals fed AH diet (P < 0.005). Contrary, protozoal numbers were not affected by the diet but were lower (P < 0.001) in faeces than in rumen. The analysis of the community structure revealed a consistently different population in structure in rumen and faeces for the three studied microbial groups and that supplementing alfalfa hay with oats led to a decrease in the similarity between sites in the rumen and faeces: similarity indexes for bacteria (57 and 27%), archaea (26 and 9%) and protozoa (62 and 22%) in animals fed AH and AHO diets, respectively.
这项工作的目的是比较山羊瘤胃和粪便样本中细菌、原生动物和古菌群落的生物量和群落结构，以及提供给它们的日粮（添加或不添加燕麦的苜蓿干草）在多大程度上产生影响。在一项交叉设计实验中，使用了4只瘤胃插管的成年山羊，实验分为两个26天的实验期，包括14天的适应期、7天的瘤胃内容物采样期和5天的消化率测量期。分别通过实时定量聚合酶链反应（qPCR）和变性梯度凝胶电泳（DGGE）评估细菌、原生动物和古菌的生物量以及群落结构。饲喂AH日粮的动物瘤胃和粪便中古菌和细菌的数量分别较高和较低（P<0.005）。相反，原生动物数量不受日粮影响，但粪便中的原生动物数量低于瘤胃（P<0.001）。群落结构分析表明，对于所研究的三个微生物组，瘤胃和粪便中的种群结构始终存在差异，并且用燕麦补充苜蓿干草会导致瘤胃和粪便中位点之间的相似性降低：饲喂AH和AHO日粮的动物中，细菌（分别为57%和27%）、古菌（分别为26%和9%）和原生动物（分别为62%和22%）的相似性指数。
