MOE Key Lab of Bioinformatics, Department of Biological Science and Biotechnology, School of Life Science, Tsinghua-Peking Center for Life Sciences, Tsinghua University, Beijing 100084, China.
Metab Eng. 2012 Jul;14(4):317-24. doi: 10.1016/j.ymben.2012.04.003. Epub 2012 Apr 27.
Copolyesters of 3-hydroxypropionate (3HP) and 4-hydroxybutyrate (4HB), abbreviated as P(3HP-co-4HB), was synthesized by Escherichia coli harboring a synthetic pathway consisting of five heterologous genes including orfZ encoding 4-hydroxybutyrate-coenzyme A transferase from Clostridium kluyveri, pcs' encoding the ACS domain of tri-functional propionyl-CoA ligase (PCS) from Chloroflexus aurantiacus, dhaT and aldD encoding dehydratase and aldehyde dehydrogenase from Pseudomonas putida KT2442, and phaC1 encoding PHA synthase from Ralstonia eutropha. When grown on mixtures of 1,3-propanediol (PDO) and 1,4-butanediol (BDO), compositions of 4HB in microbial P(3HP-co-4HB) were controllable ranging from 12 mol% to 82 mol% depending on PDO/BDO ratios. Nuclear magnetic resonance (NMR) spectra clearly indicated the polymers were random copolymers of 3HP and 4HB. Their mechanical and thermal properties showed obvious changes depending on the monomer ratios. Morphologically, P(3HP-co-4HB) films only became fully transparent when monomer 4HB content was around 67 mol%. For the first time, P(3HP-co-4HB) with adjustable monomer ratios were produced and characterized.
3-羟基丙酸（3HP）和 4-羟基丁酸（4HB）的共聚酯，缩写为 P（3HP-co-4HB），由含有由五个异源基因组成的合成途径的大肠杆菌合成，包括编码来自克氏梭菌的 4-羟基丁酸-辅酶 A 转移酶的 orfZ、来自橙色着色菌的三功能丙酰辅酶 A 连接酶（PCS）的 ACS 结构域的 pcs'、编码来自恶臭假单胞菌 KT2442 的脱水酶和醛脱氢酶的 dhaT 和 aldD，以及编码来自 Ralstonia eutropha 的 PHAsynthase 的 phaC1。当在 1,3-丙二醇（PDO）和 1,4-丁二醇（BDO）的混合物上生长时，根据 PDO/BDO 比，微生物 P（3HP-co-4HB）中 4HB 的组成可从 12 mol%到 82 mol%控制。核磁共振（NMR）谱清楚地表明，这些聚合物是 3HP 和 4HB 的无规共聚物。它们的机械和热性能根据单体比例明显变化。在形态上，只有当单体 4HB 含量约为 67 mol%时，P（3HP-co-4HB）薄膜才完全透明。首次生产和表征了具有可调节单体比例的 P（3HP-co-4HB）。
