CBQF, Escola Superior de Biotecnologia, Universidade Católica Portuguesa, Porto, Portugal.
Anaerobe. 2012 Jun;18(3):305-9. doi: 10.1016/j.anaerobe.2012.04.009. Epub 2012 Apr 27.
The objective of this study was to assess the antimicrobial capability of non-chemically altered chitosan as an alternative to traditional antimicrobials used in the treatment of oral infections. The action mechanism of chitosan was also ascertained. High and low molecular weight chitosan showed antimicrobial activity at low concentrations for all tested bacteria with the MICs varying between 1 and 7 mg/ml with a drop of efficacy relatively to the action of LMW chitosan. In addition chitosan showed also to be an effective bactericidal presenting bactericidal effect within 8 h at the latest. Additionally the evaluation of chitosan's action mechanism showed that both MWs acted upon the bacterial cell wall and were not capable of interacting with the intracellular substances, as showed by the inefficacy obtained in the flocculation assay.
本研究旨在评估未经化学修饰的壳聚糖作为替代传统口腔感染治疗用抗菌剂的抗菌能力。还确定了壳聚糖的作用机制。高分子量和低分子量壳聚糖在低浓度下对所有测试的细菌均显示出抗菌活性，MIC 值在 1 至 7 mg/ml 之间，相对于 LMW 壳聚糖的作用，其功效有所下降。此外，壳聚糖还显示出有效的杀菌作用，在 8 小时内达到杀菌效果。此外，壳聚糖作用机制的评估表明，两种分子量的壳聚糖均作用于细菌细胞壁，并且不能与细胞内物质相互作用，如絮凝试验所得到的无效结果所示。
