Department of Science Education, Jeju National University, Jeju 690-756, Republic of Korea.
Int J Syst Evol Microbiol. 2013 Feb;63(Pt 2):744-750. doi: 10.1099/ijs.0.042275-0. Epub 2012 May 4.
A strictly aerobic, Gram-stain-positive actinobacterial strain was isolated from a rhizosphere soil of a wild plant (Peucedanum japonicum Thumb.) collected on Mara Island, Jeju, Republic of Korea. Cells of strain RS-50(T) were oxidase-negative, catalase-positive, short rods and motile by means of a polar flagellum; the colonies were orange, circular, smooth and convex. meso-Diaminopimelic acid and glucose were the diagnostic diamino acid in the cell wall and the whole-cell sugar, respectively. The major menaquinone was MK-9(H(4)). The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannoside, two unknown phospholipids and an unknown lipid. The major fatty acids were iso-C(15 : 0), iso-C(16 : 0,) anteiso-C(15 : 0) and anteiso-C(17 : 0). The DNA G+C content was 73.6 mol%. In 16S rRNA gene sequence-based phylogenetic trees, strain RS-50(T) formed a tight cluster with Angustibacter luteus (99.2 % sequence similarity); both were loosely related to the suborders Kineosporiineae and Micrococcineae. The DNA-DNA relatedness value of the isolate to A. luteus KACC 14249(T) was 22.3±0.9 %. On the basis of the results of phenotypic analyses and DNA-DNA hybridization experiments, strain RS-50(T) is considered to represent a novel species of the genus Angustibacter, for which the name Angustibacter peucedani sp. nov. is proposed. The type strain is RS-50(T) ( = KCTC 19628(T) = DSM 45329(T)). The description of the genus Angustibacter is emended.
一株严格需氧、革兰氏阳性放线菌菌株，从韩国济州岛马罗岛采集的野生植物（Peucedanum japonicum Thumb.）根际土壤中分离得到。RS-50(T) 菌株的细胞为氧化酶阴性、过氧化氢酶阳性、短杆状且通过极生鞭毛运动；菌落呈橙色、圆形、光滑且凸起。meso-二氨基庚二酸和葡萄糖分别是细胞壁中的特征二氨基庚二酸和全细胞糖。主要的menaquinone 是 MK-9(H(4))。极性脂类是双磷脂酰甘油、磷脂酰甘油、磷脂酰肌醇、磷脂酰肌醇甘露糖苷、两种未知的磷脂和一种未知的脂类。主要脂肪酸是 iso-C(15 : 0)、iso-C(16 : 0)、anteiso-C(15 : 0) 和 anteiso-C(17 : 0)。DNA G+C 含量为 73.6 mol%。在基于 16S rRNA 基因序列的系统发育树中，RS-50(T) 菌株与黄杆菌属 Angustibacter luteus（99.2%序列相似性）形成紧密聚类；两者与 Kineosporiineae 和 Micrococcineae 亚目均为松散相关。该分离株与 A. luteus KACC 14249(T) 的 DNA-DNA 同源性值为 22.3±0.9%。基于表型分析和 DNA-DNA 杂交实验的结果，RS-50(T) 菌株被认为代表了 Angustibacter 属的一个新种，因此提议将其命名为 Angustibacter peucedani sp. nov.。模式菌株为 RS-50(T)（=KCTC 19628(T)=DSM 45329(T)）。对 Angustibacter 属的描述进行了修正。
