Department of Science Education, Jeju National University, Jeju 690-756, Republic of Korea.
Int J Syst Evol Microbiol. 2013 Oct;63(Pt 10):3818-3822. doi: 10.1099/ijs.0.052357-0. Epub 2013 May 10.
A rod-shaped actinobacterium, designated strain RS-16(T), was isolated from a rhizosphere soil and its taxonomic position was determined by a polyphasic taxonomic approach. Comparative 16S rRNA gene sequence analysis indicated that strain RS-16(T) was most closely related to the type strain of Motilibacter peucedani (98.3 % sequence similarity). The cell-wall peptidoglycan contained LL-diaminopimelic acid as the diagnostic diamino acid and N-glycolylated murein. The major whole-cell sugars were glucose, galactose and mannose. The predominant menaquinone was MK-9(H4). The major fatty acids were C18 : 1ω9c and C16 : 0. The DNA G+C content was 73.1 mol%. The phenotypic and DNA-DNA hybridization data showed that strain RS-16(T) ( = KACC 16209(T) = DSM 45622(T)) represents a novel species of the genus Motilibacter, for which Motilibacter rhizosphaerae sp. nov. is proposed. Based on the phylogenetic position determined by 16S rRNA gene analysis and the signature nucleotide set of the 16S rRNA sequence, the genus Motilibacter represents a novel family of the suborder Frankineae, for which the name Motilibacteraceae fam. nov. is proposed.
一株棒状放线菌，命名为 RS-16(T) 菌株，从根际土壤中分离得到，通过多相分类学方法确定了其分类地位。比较 16S rRNA 基因序列分析表明，RS-16(T) 菌株与 Motilibacter peucedani 的模式菌株最为密切相关（98.3%序列相似性）。细胞壁肽聚糖含有 LL-二氨基庚二酸作为诊断二氨基酸和 N-糖基化的壁酸。主要的全细胞糖是葡萄糖、半乳糖和甘露糖。主要的类异戊二烯醌是 MK-9(H4)。主要脂肪酸是 C18:1ω9c 和 C16:0。DNA G+C 含量为 73.1 mol%。表型和 DNA-DNA 杂交数据表明，RS-16(T) 菌株（=KACC 16209(T)=DSM 45622(T)）代表了 Motilibacter 属的一个新种，建议将其命名为 Motilibacter rhizosphaerae sp. nov.。基于 16S rRNA 基因分析确定的系统发育位置和 16S rRNA 序列的特征核苷酸集，Motilibacter 属代表了 Frankineae 亚目的一个新科，建议命名为 Motilibacteraceae fam. nov.。
