González del Pino J, Benito M, Randolph M A, Weiland A J
Orthopaedic Research Laboratories, Johns Hopkins Hospital, Baltimore, Maryland 21205.
Transplantation. 1990 Dec;50(6):920-7. doi: 10.1097/00007890-199012000-00003.
To evaluate the effects of irradiation on heterotopically placed vascularized knee isografts, a single dose of 10 Gy of total-body irradiation was given to Lewis donor rats. Irradiation was delivered either 2 or 6 days prior to harvesting or subsequent transplantation, and evaluated at 1, 2, and 4 weeks after grafting. Irradiation caused endothelial depopulation of the graft artery, although vascular pedicle patency was maintained throughout the study. Bone graft viability and mineralization were normal. Dramatic changes in the bone marrow were seen that included an increase of its fat content (P less than 0.001), and a concomitant decrease in bone marrow-derived immunocompetent cells. These changes were more prominent in recipients of grafts from day -6 irradiated donor rats. Total-body irradiation did not prejudice the use of vascularized bone grafts, and exhibited an associated immunosuppresant effect over the vascular endothelium and bone marrow. This may be a further rational conditioning procedure to avoid recipient manipulation in vascularized bone allotransplantation.
为评估辐射对异位放置的带血管膝关节同种异体移植物的影响，对Lewis供体大鼠进行了单次10 Gy的全身照射。在收获或随后移植前2天或6天进行照射，并在移植后1周、2周和4周进行评估。尽管在整个研究过程中血管蒂保持通畅，但照射导致移植动脉的内皮细胞减少。骨移植的活力和矿化正常。观察到骨髓有显著变化，包括脂肪含量增加（P<0.001），以及骨髓来源的免疫活性细胞随之减少。这些变化在接受来自第6天照射的供体大鼠移植物的受体中更为明显。全身照射不影响带血管骨移植的使用，并且对血管内皮和骨髓表现出相关的免疫抑制作用。这可能是避免在带血管骨同种异体移植中对受体进行操作的进一步合理预处理程序。
