Gonzalez del Pino J, Benito M, Randolph M A, Weiland A J
Orthopaedic Research Laboratory, Johns Hopkins University School of Medicine, Baltimore, Maryland.
J Reconstr Microsurg. 1990 Jul;6(3):229-37. doi: 10.1055/s-2007-1006823.
At the present time, the toxic side effects of recipient immunosuppression cannot be justified for human non-vital organ transplantation. Total body irradiation has proven effective in ablating various bone-marrow-derived and endothelial immunocompetent cellular populations, which are responsible for immune rejection against donor tissues. Irradiation at a dose of 10 Gy was given to donor rats six days prior to heterotopic transplantation of vascularized bone allografts to host animals. Another group of recipient rats also received a short-term (sixth to fourteenth day after grafting), low dose of cyclosporine. Total body irradiation was able merely to delay rejection of grafts across a strong histocompatibility barrier for one to two weeks, when compared to nonirradiated allografts. The combination of donor irradiation plus cyclosporine did not delay the immune response, and the rejection score was similar to that observed for control allografts. Consequently, allograft viability was quickly impaired, leading to irreversible bone damage. This study suggest that 10 Gy of donor total body irradiation delivered six days prior to grafting cannot circumvent the immune rejection in a vascularized allograft of bone across a strong histocompatibility barrier.
目前，对于人体非重要器官移植而言，受体免疫抑制带来的毒副作用尚无合理依据。全身照射已被证明能有效消除各种骨髓来源和内皮免疫活性细胞群体，这些细胞群体是导致对供体组织产生免疫排斥反应的原因。在将血管化同种异体骨移植到宿主动物的异位移植前六天，对供体大鼠进行10 Gy的照射。另一组受体大鼠也接受了短期（移植后第六天至第十四天）低剂量的环孢素。与未照射的同种异体移植相比，全身照射仅能将跨越强组织相容性屏障的移植物排斥反应延迟一至两周。供体照射加环孢素的联合使用并未延迟免疫反应，排斥评分与对照同种异体移植观察到的相似。因此，同种异体移植物的活力很快受损，导致不可逆的骨损伤。本研究表明，在移植前六天进行10 Gy的供体全身照射，无法在跨越强组织相容性屏障的血管化同种异体骨移植中规避免疫排斥反应。
