Biomolecular Mass Spectrometry and Proteomics, Bijvoet Center for Biomolecular Research and Utrecht Institute of Pharmaceutical Sciences, Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands.
J Proteomics. 2012 Jul 16;75(13):3791-813. doi: 10.1016/j.jprot.2012.04.033. Epub 2012 May 4.
Shotgun proteomics dominates the field of proteomics. The foundations of the strategy consist of multiple rounds of peptide separation where chromatography provides the bedrock. Initially, the scene was relatively simple with the majority of strategies based on some types of ion exchange and reversed phase chromatography. The thirst to achieve comprehensivity, when it comes to proteome coverage and the global characterization of post translational modifications, has led to the introduction of several new separations. In this review, we attempt to provide a historical perspective to separations in proteomics as well as indicate the principles of their operation and rationales for their implementation. Furthermore, we provide a guide on what are the possibilities for combining different separations in order to increase peak capacity and proteome coverage. We aim to show how separations enrich the world of proteomics and how further developments may impact the field.
shotgun 蛋白质组学主导着蛋白质组学领域。该策略的基础包括多轮肽分离，其中色谱法提供了基础。最初，情况相对简单，大多数策略基于某些类型的离子交换和反相色谱法。当涉及到蛋白质组覆盖范围和翻译后修饰的全面描述时，对全面性的渴望导致了几种新分离方法的引入。在这篇综述中，我们试图提供蛋白质组学中分离的历史视角，以及指示它们的操作原理和实施理由。此外，我们还提供了一个指南，说明如何组合不同的分离方法以增加峰容量和蛋白质组覆盖范围。我们旨在展示分离方法如何丰富蛋白质组学的世界，以及进一步的发展可能会如何影响该领域。
