Key Laboratory of Separation Science for Analytical Chemistry, National Chromatographic Research and Analysis Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, China.
Anal Chim Acta. 2012 Jun 20;731:1-10. doi: 10.1016/j.aca.2012.04.010. Epub 2012 Apr 21.
With the acceleration of proteome research, increasing attention has been paid to multidimensional liquid chromatography-mass spectrometry (MDLC-MS) due to its high peak capacity and separation efficiency. Recently, many efforts have been put to improve MDLC-based strategies including "top-down" and "bottom-up" to enable highly sensitive qualitative and quantitative analysis of proteins, as well as accelerate the whole analytical procedure. Integrated platforms with combination of sample pretreatment, multidimensional separations and identification were also developed to achieve high throughput and sensitive detection of proteomes, facilitating highly accurate and reproducible quantification. This review summarized the recent advances of such techniques and their applications in qualitative and quantitative analysis of proteomes.
随着蛋白质组学研究的加速，多维液相色谱-质谱联用（MDLC-MS）因其高峰容量和分离效率而受到越来越多的关注。最近，人们做出了许多努力来改进基于 MDLC 的策略，包括“自上而下”和“自下而上”的方法，以实现对蛋白质的高灵敏度定性和定量分析，并加速整个分析过程。集成的平台与样品预处理、多维分离和鉴定相结合，也被开发出来，以实现蛋白质组的高通量和灵敏检测，从而促进高度准确和可重复的定量。本文综述了这些技术的最新进展及其在蛋白质组定性和定量分析中的应用。
