Division of Agronomy and Soil Science, Central Institute of Medicinal and Aromatic Plants, P.O. CIMAP, Lucknow 226015, India.
J Microbiol Biotechnol. 2012 May;22(5):674-83. doi: 10.4014/jmb.1109.09061.
A new strain, SD12, was isolated from tannery waste polluted soil and identified as Pseudomonas aeruginosa on the basis of phenotypic traits and by comparison of 16S rRNA sequences. This bacterium exhibited broad-spectrum antagonistic activity against phytopathogenic fungi. The strain produced phosphatases, cellulases, proteases, pectinases, and HCN and also retained its ability to produce hydroxamate-type siderophore. A bioactive metabolite was isolated from P. aeruginosa SD12 and was characterized as 1-hydroxyphenazine ((1-OH-PHZ) by nuclear magnetic resonance (NMR) spectral analysis. The strain was used as a biocontrol agent against root rot and wilt disease of pyrethrum caused by Rhizoctonia solani. The stain is also reported to increase the growth and biomass of Plantago ovata. The purified compound, 1-hydroxyphenazine, also showed broad-spectrum antagonistic activity towards a range of phytopathogenic fungi, which is the first report of its kind.
一株新的 SD12 菌株从制革厂污染土壤中分离出来，根据表型特征和 16S rRNA 序列的比较，鉴定为铜绿假单胞菌。该细菌对植物病原真菌表现出广谱拮抗活性。该菌株产生磷酸酶、纤维素酶、蛋白酶、果胶酶和 HCN，并且仍然能够产生羟肟酸型铁载体。从铜绿假单胞菌 SD12 中分离到一种生物活性代谢物，并通过核磁共振（NMR）谱分析鉴定为 1-羟基吩嗪（1-OH-PHZ）。该菌株被用作生物防治剂，防治万寿菊根腐病和立枯丝核菌引起的枯萎病。该菌株还被报道可以增加车前草的生长和生物量。纯化的化合物 1-羟基吩嗪也对一系列植物病原真菌表现出广谱拮抗活性，这是此类化合物的首次报道。
