Department of Veterinary Medicine Virology Laboratory College of Veterinary Medicine and BK21 Program for Veterinary Science, Seoul National University, Seoul 151-742, Korea.
J Microbiol Biotechnol. 2012 May;22(5):699-707. doi: 10.4014/jmb.1106.05062.
Since the 2009 pandemic human H1N1 influenza A virus emerged in April 2009, novel reassortant strains have been identified throughout the world. This paper describes the detection and isolation of reassortant strains associated with human pandemic influenza H1N1 and swine influenza H1N2 (SIV) viruses in swine populations in South Korea. Two influenza H1N2 reassortants were detected, and subtyped by PCR. The strains were isolated using Madin- Darby canine kidney (MDCK) cells, and genetically characterized by phylogenetic analysis for genetic diversity. They consisted of human, avian, and swine virus genes that were originated from the 2009 pandemic H1N1 virus and a neuraminidase (NA) gene from H1N2 SIV previously isolated in North America. This identification of reassortment events in swine farms raises concern that reassortant strains may continuously circulate within swine populations, calling for the further study and surveillance of pandemic H1N1 among swine.
自 2009 年 4 月甲型 H1N1 流感大流行以来，世界各地已发现新型重组株。本文描述了在韩国猪群中与人甲型 H1N1 流感和猪源甲型 H1N2 流感（SIV）病毒相关的重组株的检测和分离。通过 PCR 检测和亚型鉴定，发现了两种流感 H1N2 重组株。这些毒株通过 Madin-Darby 犬肾（MDCK）细胞进行分离，并通过遗传多样性的系统进化分析进行遗传特征分析。它们由源自 2009 年大流行 H1N1 病毒的人、禽和猪病毒基因以及先前在北美分离的 H1N2 SIV 的神经氨酸酶（NA）基因组成。在养猪场中发现重组事件令人担忧，因为重组株可能会在猪群中持续循环，需要进一步研究和监测猪群中的大流行 H1N1 病毒。
