Bagher Zohreh, Rajaei Farzad, Shokrgozar Mohammadali
Cellular and Molecular Research Centre, Qazvin University of Medical Sciences, Qazvin, Iran.
National Cell Bank of Iran, Pasteur Institute of Iran, Tehran, Iran.
Iran Biomed J. 2012;16(1):18-24. doi: 10.6091/ibj.996.2012.
Bone tissue engineering requires materials that are biocompatible, mechanically suited for bone function, integrated with the host skeleton, and support osteoinduction of the implanted cells for new bone formation. The aim of this study was to compare the osteogenic potential of xenograft with hydroxyapatite/β- tricalcium phosphate (HA/β-TCP) scaffold.
New Zealand rabbits (n = 9) were divided into 3 groups. Osteoblast cells were originally isolated from rabbit iliac crest and cultured in DMEM/F12. After creating a critical-sized defect (2 × 3 cm) in rabbit tibia bone, the defect was filled with an implant of HA/TCP with osteoblasts and xenograft in the hole of left (as control) and right tibia, respectively. The new bone formation and the development of bone union within the defect were evaluated by x-ray images and eosine and hematoxylin staining at 4, 8, and 12 weeks post-operation.
The bone partially formed in both groups was filled with osteoblast cultured on porous implants at 4 weeks. Over time, progressive bone regeneration was observed inside the pores. Moreover, a progressive vascular ingrowth and progressive integration with the host bone were obvious in xenograft when compared to HA/β-TCP. A good integration between the xenograft implants and the bone was observed radiographically and confirmed by histological section.
The result showed that the bone defect can be repaired using both synthetic and xenograft implants. However, the xenograft showed a better osteointegration as compared to HA/β-TCP scaffold.
骨组织工程需要具有生物相容性、力学性能适合骨功能、能与宿主骨骼整合并支持植入细胞骨诱导以形成新骨的材料。本研究的目的是比较异种移植物与羟基磷灰石/β-磷酸三钙（HA/β-TCP）支架的成骨潜力。
将9只新西兰兔分为3组。成骨细胞最初从兔髂嵴分离并在DMEM/F12中培养。在兔胫骨上制造一个临界尺寸的缺损（2×3厘米）后，分别在左胫骨（作为对照）和右胫骨的孔中植入带有成骨细胞的HA/TCP植入物和异种移植物来填充缺损。在术后4周、8周和12周通过X线图像以及伊红和苏木精染色评估缺损内新骨形成和骨愈合情况。
术后4周时，两组中部分形成的骨均充满了在多孔植入物上培养的成骨细胞。随着时间推移，在孔隙内观察到渐进性骨再生。此外，与HA/β-TCP相比，异种移植物中明显可见渐进性血管长入以及与宿主骨的渐进性整合。通过X线检查观察到异种移植物植入物与骨之间有良好的整合，并经组织学切片证实。
结果表明，使用合成植入物和异种移植物均可修复骨缺损。然而，与HA/β-TCP支架相比，异种移植物显示出更好的骨整合。
