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在吲哚乙酸、表皮生长因子和促卵泡激素存在的情况下培养的绵羊原始卵泡的超微结构

Ultrastructure of Sheep Primordial Follicles Cultured in the Presence of Indol Acetic Acid, EGF, and FSH.

作者信息

Andrade Evelyn Rabelo, Maddox-Hyttel Poul, Landim-Alvarenga Fernanda Da Cruz, Viana Silva José Roberto, Alfieri Amauri Alcindo, Seneda Marcelo Marcondes, Figueiredo José Ricardo, Toniolli Ricardo

机构信息

Universidade Estadual de Londrina, DCV-CCA-UEL, 86051-990, Londrina, PR, Brazil.

出版信息

Vet Med Int. 2010 Dec 6;2011:670987. doi: 10.4061/2011/670987.

DOI:10.4061/2011/670987
PMID:21188166
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3005833/
Abstract

The aim of this study was to investigate the ultrastructural characteristics of primordial follicles after culturing of sheep ovarian cortical slices in the presence of indol acetic acid (IAA), Epidermal Growth Factor (EGF), and FSH. To evaluate ultrastructure of primordial follicles cultured in MEM (control) or in MEM containing IAA, EGF, and FSH, fragments of cultured tissue were processes for transmission electron microscopy. Except in the control, primordial follicles cultured in supplemented media for 6 d were ultrastructurally normal. They had oocyte with intact nucleus and the cytoplasm contained heterogeneous-sized lipid droplets and numerous round or elongated mitochondria with intact parallel cristae were observed. Rough endoplasmic reticulum (RER) was rarely found. The granulosa cells cytoplasm contained a great number of mitochondria and abundant RER. In conclusion, the presence of IAA, EGF, and FSH helped to maintain ultrastructural integrity of sheep primordial follicles cultured in vitro.

摘要

本研究的目的是探讨在吲哚乙酸（IAA）、表皮生长因子（EGF）和促卵泡激素（FSH）存在的情况下培养绵羊卵巢皮质切片后原始卵泡的超微结构特征。为了评估在MEM（对照）或含有IAA、EGF和FSH的MEM中培养的原始卵泡的超微结构，将培养组织的片段进行透射电子显微镜处理。除对照外，在补充培养基中培养6天的原始卵泡超微结构正常。它们的卵母细胞核完整，细胞质中含有大小不一的脂滴，观察到许多圆形或细长的线粒体，其嵴完整且平行。很少发现粗面内质网（RER）。颗粒细胞的细胞质中含有大量线粒体和丰富的RER。总之，IAA、EGF和FSH的存在有助于维持体外培养的绵羊原始卵泡的超微结构完整性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9175/3005833/c9f7c7b4bdf0/VMI2011-670987.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9175/3005833/bfd086832dcd/VMI2011-670987.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9175/3005833/8a0a13ab2470/VMI2011-670987.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9175/3005833/c9f7c7b4bdf0/VMI2011-670987.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9175/3005833/bfd086832dcd/VMI2011-670987.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9175/3005833/8a0a13ab2470/VMI2011-670987.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9175/3005833/c9f7c7b4bdf0/VMI2011-670987.003.jpg

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