Division of Gastroenterology, Department of Medicine, Taipei Veterans General Hospital, Taipei, Taiwan.
Mol Med Rep. 2012 Jul;6(1):191-6. doi: 10.3892/mmr.2012.901. Epub 2012 May 3.
Obestatin, which is a putative 23-amino-acid peptide, is derived from the C-terminal part of the mammalian preproghrelin gene. Nesfatin-1 mRNA is co-expressed with ghrelin in gastric endocrine X/A-like cells; therefore, nesfatin-1 may also interact with preproghrelin gene products in the stomach. In this study, we investigated the impact of obestatin on the plasma levels of acyl ghrelin, des-acyl ghrelin and nesfatin-1, and on the gastric emptying of a solid nutrient meal 2 h after an intracerebroventricular (ICV) injection in conscious, fasted rats. The rats were implanted with ICV catheters. Plasma levels of acyl ghrelin, des-acyl ghrelin and nesfatin-1, expected to be co-expressed with obestatin, were measured, whereas the human/rat corticotropin-releasing factor (h/rCRF) was applied as an inhibitor of gastric emptying. The ICV administration of obestatin (0.1, 0.3 and 1.0 nmol/rat) did not modify the plasma acyl ghrelin and des-acyl ghrelin levels, the acyl ghrelin/des-acyl ghrelin ratio and nesfatin-1 concentrations. The ICV acute administration of obestatin had no influence on the 2-h rate of gastric emptying of a solid nutrient meal, but the ICV h/rCRF injection delayed it. The weight of food ingested 1 h before ICV injection significantly, but negatively correlated with the gastric emptying of a solid nutrient meal. Our study indicates that the ICV injection of obestatin does not change the 2-h rate of gastric emptying of a solid nutrient meal and the relatively weak interrelationships between ghrelin gene products and nesfatin-1. However, the weight of the ingested food negatively affects the gastric emptying of a solid nutrient meal in conscious, fasted rats.
肥胖抑制素是一种假定的 23 个氨基酸肽，源自哺乳动物前胃饥饿素基因的 C 端部分。Nesfatin-1mRNA 与胃内分泌 X/A 样细胞中的胃饥饿素共表达；因此，nesfatin-1 也可能与胃中的前胃饥饿素基因产物相互作用。在这项研究中，我们研究了肥胖抑制素对酰基胃饥饿素、脱酰基胃饥饿素和 nesfatin-1 的血浆水平以及清醒、禁食大鼠脑室内（ICV）注射后 2 小时固体营养餐后胃排空的影响。大鼠被植入了 ICV 导管。测量了酰基胃饥饿素、脱酰基胃饥饿素和 nesfatin-1 的血浆水平，预计这些激素与肥胖抑制素共表达，而人类/大鼠促肾上腺皮质释放因子（h/rCRF）被用作胃排空的抑制剂。ICV 给予肥胖抑制素（0.1、0.3 和 1.0nmol/大鼠）并未改变酰基胃饥饿素和脱酰基胃饥饿素水平、酰基胃饥饿素/脱酰基胃饥饿素比值和 nesfatin-1 浓度。ICV 急性给予肥胖抑制素对固体营养餐后 2 小时胃排空率没有影响，但 ICV h/rCRF 注射延迟了胃排空。ICV 注射前 1 小时摄入的食物重量显著但负相关与固体营养餐后的胃排空。我们的研究表明，ICV 注射肥胖抑制素不会改变固体营养餐后 2 小时的胃排空率，以及胃饥饿素基因产物和 nesfatin-1 之间的相对较弱的相互关系。然而，摄入食物的重量会负影响清醒、禁食大鼠固体营养餐后的胃排空。
