Laboratory of Protozoology, Institute of Evolution and Marine Biodiversity, Ocean University of China, Qingdao 266003, China.
Environ Sci Pollut Res Int. 2012 Sep;19(8):3619-27. doi: 10.1007/s11356-012-0938-5. Epub 2012 May 5.
Colonization features and taxonomic relatedness measures of ciliate communities have been used as useful indicators for marine bioassessment. The influence of enumeration time periods on analyzing colonization features measures of periphytic ciliate communities was studied in coastal waters of the Yellow Sea, northern China, during the period of May-June 2010. Ciliated protozoan samples were collected at depths of 1 m using an artificial substratum and were analyzed with different enumeration schemes. The ciliate species were identified using living observation and silver impregnation. Data analyses were conducted using a range of multivariate statistical routines. Enumeration time periods represented a strong influence on analyzing both colonization and taxonomic relatedness features of periphytic ciliate communities, although no significant changes occurred in colonization patterns between two enumeration schemes (within 24 and 24-48 h after sampling). The delayed enumeration (within 24-48 h) may result in the species richness, individual abundance, colonization rate decreasing to standard errors of >10 % in samples with almost all colonization ages, and in the similarities of the communities being reduced to 11-38 %. However, the species biodiversity (e.g., species diversity and evenness, except species richness) and taxonomic relatedness (taxonomic diversity, taxonomic distinctness and average taxonomic distinctness, except variation in taxonomic distinctness) measures of periphytic ciliate communities were weakly sensitive to disturbance from the delayed enumeration, achieving standard errors of <10 and <5 % during the colonization periods, respectively. These results suggest that the enumeration should be completed as soon as possible within 24 h after sampling to analyze colonization and taxonomic relatedness features of periphytic ciliate communities, and that the species diversity and taxonomic distinctness measures can be used on a robust bioindicator with weak dependence on enumeration time limits for monitoring programs and ecological investigations in marine ecosystems.
纤毛虫群落的定殖特征和分类相关度指标已被用作海洋生物评估的有用指标。本研究于 2010 年 5-6 月在中国黄海沿海地区，通过使用人工基质在 1 m 水深处采集样品，采用不同的计数方案，研究了纤毛虫定殖特征分析中计数时间周期的影响。使用活体观察和银染技术对纤毛虫样本进行鉴定。采用一系列多元统计程序进行数据分析。尽管在两种计数方案（采样后 24 和 24-48 小时内）之间没有发生明显的定殖模式变化，但计数时间周期对分析附着生物纤毛虫群落的定殖和分类相关特征具有强烈的影响。延迟计数（采样后 24-48 小时内）可能导致物种丰富度、个体丰度、定殖率在几乎所有定殖龄的样品中降低到>10%的标准误差，而群落的相似性降低到 11-38%。然而，附着生物纤毛虫群落的物种生物多样性（例如，物种多样性和均匀度，除了物种丰富度）和分类相关度（分类多样性、分类独特度和平均分类独特度，除了分类独特度的变化）测量值对延迟计数的干扰较弱，在定殖期间分别达到<10%和<5%的标准误差。这些结果表明，为了分析附着生物纤毛虫群落的定殖和分类相关特征，应在采样后 24 小时内尽快完成计数，并且物种多样性和分类独特度指标可以作为一种稳健的生物指标，对监测计划和海洋生态系统的生态调查具有较弱的计数时间限制依赖性。
