Key Laboratory of Horticultural Plant Biology of Ministry of Education, Huazhong Agricultural University, Wuhan 430070, China.
Mol Biol Rep. 2012 Aug;39(8):8187-95. doi: 10.1007/s11033-012-1666-0. Epub 2012 May 5.
Chestnut rose (Rosa roxburghii Tratt), a non-domesticated potential fruit crop, is susceptible to infection by powdery mildew. A small-scale customized macro-array was assembled with cDNA clones from a suppression subtractive hybridization library enriched for defense transcripts and used to screen for differentially expressed genes induced by powdery mildew infection. Three photorespiratory genes, encoding the small subunit of RuBisCO (ribulose-1,5-bisphosphate carboxylase/oxygenase), RuBisCO activase and serine-glyoxylate aminotransferase, were identified in this screen. Quantitative RT-PCR analysis of these three genes showed that they were significantly up-regulated 16 h after the host was inoculated with powdery mildew, and assays at the enzyme level confirmed high levels of enzymatic activity 24 h after infection. Of six phytohormones tested, salicylic acid (SA) noticeably induced their expression, and HPLC analysis showed that SA accumulated in the leaves after fungal infection.
栗玫瑰（Rosa roxburghii Tratt）是一种非驯化的潜在水果作物，易受白粉病感染。利用抑制差减杂交文库中富含防御转录物的 cDNA 克隆组装了一个小规模定制的宏阵列，用于筛选白粉病感染诱导的差异表达基因。在该筛选中鉴定出三个光呼吸基因，分别编码 RuBisCO（核酮糖-1,5-二磷酸羧化酶/加氧酶）的小亚基、RuBisCO 激活酶和丝氨酸-乙醛酸氨基转移酶。对这三个基因的定量 RT-PCR 分析表明，它们在宿主被白粉病接种后 16 小时显著上调，并且在感染 24 小时后在酶水平上证实了高水平的酶活性。在测试的六种植物激素中，水杨酸（SA）明显诱导了它们的表达，HPLC 分析表明，在真菌感染后，SA 在叶片中积累。
