Key Laboratory of Plant Resources, Institute of Botany, Chinese Academy of Sciences, Xiangshan, Beijing, People's Republic of China.
Curr Microbiol. 2012 Aug;65(2):122-7. doi: 10.1007/s00284-012-0133-4. Epub 2012 May 6.
In this article, we investigated the effect of exogenous calcium on improving viability of Debaryomyces hansenii and Pichia membranaefaciens under heat stress, and evaluated the role of calcium in reducing oxidant damage of proteins in the yeast cells. The results indicated that high concentration of exogenous calcium in culture medium was beneficial for enhancing the tolerance of the biocontrol yeasts to heat stress. The possible mechanism of calcium improving the viability of yeasts was attributed to enhancement of antioxidant enzyme activities, decrease in ROS accumulation and reduction of oxidative damage of intracellular protein in yeast cells under heat stress. D. hansenii is more sensitive to calcium as compared to P. membranaefaciens. Our results suggest that application of exogenous calcium combined with biocontrol yeasts is a practical approach for the control of postharvest disease in fruit.
在本文中，我们研究了外源钙对提高耐热德巴利氏酵母和膜醭毕赤酵母活力的影响，并评估了钙在降低酵母细胞中蛋白质氧化剂损伤中的作用。结果表明，培养基中高浓度的外源钙有利于增强生防酵母对热应激的耐受性。钙提高酵母活力的可能机制归因于增强抗氧化酶活性、减少 ROS 积累以及降低热应激下酵母细胞内蛋白质的氧化损伤。与膜醭毕赤酵母相比，德巴利氏酵母对钙更为敏感。我们的结果表明，外源钙与生防酵母联合应用是控制水果采后病害的一种实用方法。
