Zhang Weiwei, Zhuang Shunyao, Gao Jianshuang
State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Nanjing, China.
University of the Chinese Academy of Sciences, Beijing, China.
Front Microbiol. 2025 May 9;16:1545248. doi: 10.3389/fmicb.2025.1545248. eCollection 2025.
The biocontrol efficiency of the antagonist yeast is significantly reduced under oxidative stress in adverse environments. However, effective strategies to improve under such abiotic stress remain limited. As an effective protectant of yeasts, xylitol has significant potential to improve the performance of under abiotic stress. We investigated xylitol's effects on the viability and efficiency of under oxidative stress. The results showed that 0.5 M and 1 M xylitol significantly enhanced yeast survival, antioxidant gene expression, and enzyme activity, including thioredoxin reductase (TrxR) and peroxidase (POD), while reducing intracellular reactive oxygen species levels as well as damage to mitochondrial membranes, and preserving the ATP content. Notably, xylitol-treated (XT) yeast exhibited higher intracellular xylitol levels and improved resistance to oxidative stress compared with the non-xylitol-treated cells. Additionally, XT yeast showed a greater biocontrol efficacy and lower postharvest fungal infection rate by gray mold and blue mold in apples. These results demonstrated that xylitol effectively boosts the resilience and biocontrol efficiency of , making it a promising candidate to improve postharvest disease management.
在不利环境下的氧化应激条件下，拮抗菌酵母的生物防治效率会显著降低。然而，在这种非生物胁迫下提高其效率的有效策略仍然有限。作为酵母的一种有效保护剂，木糖醇在提高酵母在非生物胁迫下的性能方面具有巨大潜力。我们研究了木糖醇在氧化应激下对酵母活力和效率的影响。结果表明，0.5 M和1 M的木糖醇显著提高了酵母的存活率、抗氧化基因表达和酶活性，包括硫氧还蛋白还原酶（TrxR）和过氧化物酶（POD），同时降低了细胞内活性氧水平以及对线粒体膜的损伤，并保持了ATP含量。值得注意的是，与未用木糖醇处理的细胞相比，用木糖醇处理（XT）的酵母表现出更高的细胞内木糖醇水平和更强的抗氧化应激能力。此外，XT酵母对苹果采后灰霉病和青霉病的生物防治效果更好，采后真菌感染率更低。这些结果表明，木糖醇有效地提高了酵母的恢复力和生物防治效率，使其成为改善采后病害管理的一个有前景的候选物质。
