Institut de Biologie et Chimie des Protéines, FRE 3310, Dysfonctionnements de l'homéostasie tissulaire et ingénierie thérapeutique, (DyHTIT), 7 passage du Vercors, 69367, Lyon, France.
Amino Acids. 2012 Nov;43(5):2153-63. doi: 10.1007/s00726-012-1300-4. Epub 2012 May 5.
The role of polyamines in renal physiology is only partially understood. Moreover, most of the data on the enzymes of polyamine metabolism come from studies using whole kidneys. The aim of the present study was to analyze the mRNA abundance of the genes implicated in both the polyamine biosynthetic and catabolic pathways in different renal zones of male and female mice, by means of the quantitative reverse transcription-polymerase chain reaction. Our results indicate that there is an uneven distribution of the different mRNAs studied in the five renal zones: superficial cortex, deep cortex, outer stripe of the outer medulla (OS), inner stripe of the outer medulla (IS), and the inner medulla + papilla (IM). The biosynthetic genes, ornithine decarboxylase (ODC) and spermine synthase, were more expressed in the cortex, whereas the mRNAs of the catabolic genes spermine oxidase (SMO) and diamine oxidase were more abundant in IS and IM. The genes involved in the regulation of polyamine synthesis (AZ1, AZ2 and AZIN1) were expressed in all the renal zones, predominantly in the cortex, while AZIN2 gene was more abundant in the OS. ODC, SMO, spermidine synthase and spermidine/spermine acetyl transferase expression was higher in males than in females. In conclusion, the genes encoding for the polyamine metabolism were specifically and quantitatively distributed along the corticopapillary axis of male and female mouse kidneys, suggesting that their physiological role is essential in defined renal zones and/or nephron segments.
多胺在肾脏生理中的作用尚未完全阐明。此外，大多数关于多胺代谢酶的研究数据来自于使用整个肾脏的研究。本研究的目的是通过定量逆转录-聚合酶链反应分析雄性和雌性小鼠不同肾区参与多胺生物合成和分解代谢途径的基因的 mRNA 丰度。我们的研究结果表明，在所研究的 5 个肾区（外皮质、内皮质、外髓外带（OS）、外髓内带（IS）和内髓+乳头）中，不同的 mRNA 分布不均匀。生物合成基因鸟氨酸脱羧酶（ODC）和精脒合酶在皮质中表达较多，而代谢基因精脒氧化酶（SMO）和二胺氧化酶的 mRNA 在 IS 和 IM 中更丰富。多胺合成调控基因（AZ1、AZ2 和 AZIN1）在所有肾区均有表达，主要在皮质中，而 AZIN2 基因在 OS 中更丰富。ODC、SMO、精脒合酶和精脒/精脒乙酰转移酶在雄性中的表达高于雌性。综上所述，编码多胺代谢的基因在雄性和雌性小鼠肾脏的皮质-乳头轴上特异性和定量分布，表明其生理作用在特定的肾区和/或肾单位段是必不可少的。
