Bettuzzi S, Marinelli M, Strocchi P, Davalli P, Cevolani D, Corti A
Dipartimento di Scienze Biomediche, Università di Modena, Italy.
FEBS Lett. 1995 Dec 27;377(3):321-4. doi: 10.1016/0014-5793(95)01359-8.
In situ hybridization histochemistry of transverse sections from male rat kidney showed that the mRNA of the regulatory enzyme of polyamine degradation, spermidine/spermine N1-acetyltransferase, has a spotty distribution in the cortex, is low and diffused in the outer stripe and high and diffused in the inner stripe of the outer medulla. At the cellular level, this mRNA is solely expressed by the epithelium of the distal straight and convoluted nephron tubules. Since biosynthetic ornithine decarboxylase mRNA is solely found in the proximal straight tubules, it is proposed that polyamine biosynthesis and degradation occur at separate sites along the nephron.
雄性大鼠肾脏横切片的原位杂交组织化学显示，多胺降解调节酶亚精胺/精胺N1 - 乙酰转移酶的mRNA在皮质呈斑点状分布，在外髓质的外带低且弥散，在内带高且弥散。在细胞水平上，这种mRNA仅由远端直小管和曲小管的上皮细胞表达。由于生物合成鸟氨酸脱羧酶mRNA仅在近端直小管中发现，因此有人提出多胺的生物合成和降解发生在肾单位的不同部位。
