筛选可有效中和副溶血性弧菌不耐热溶血素细胞毒性的单链可变片段抗体。
Screening for a single-chain variable-fragment antibody that can effectively neutralize the cytotoxicity of the Vibrio parahaemolyticus thermolabile hemolysin.
机构信息
The Ministry of Education Key Laboratory of Biopesticide and Chemical Biology and the College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, China.
出版信息
Appl Environ Microbiol. 2012 Jul;78(14):4967-75. doi: 10.1128/AEM.00435-12. Epub 2012 May 4.
Abstract
Vibrio parahaemolyticus is a halophilic bacterium that is widely distributed in water resources. The bacterium causes lethal food-borne diseases and poses a serious threat to human and animal health all over the world. The major pathogenic factor of V. parahaemolyticus is thermolabile hemolysin (TLH), encoded by the tlh gene, but its toxicity mechanisms are unknown. A high-affinity antibody that can neutralize TLH activity effectively is not available. In this study, we successfully expressed and purified the TLH antigen and discovered a high-affinity antibody to TLH, named scFv-LA3, by phage display screening. Cytotoxicity analysis showed that scFv-LA3 has strong neutralization effects on TLH-induced cell toxicity.
摘要
副溶血性弧菌是一种广布于水资源中的嗜盐细菌。该细菌会引起致命的食源性疾病，对世界各地的人类和动物健康构成严重威胁。副溶血性弧菌的主要致病因子是不耐热溶血素（TLH），由 tlh 基因编码，但它的毒性机制尚不清楚。目前还没有能够有效中和 TLH 活性的高亲和力抗体。在本研究中，我们通过噬菌体展示筛选成功表达和纯化了 TLH 抗原，并发现了一种针对 TLH 的高亲和力抗体，命名为 scFv-LA3。细胞毒性分析表明，scFv-LA3 对 TLH 诱导的细胞毒性具有很强的中和作用。
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