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[豚鼠胰腺中的胰蛋白酶和α-淀粉酶活性。III. 猪蛔虫侵袭性虫卵匀浆和C2-C6有机酸对胰腺提取物中酶活性的影响]

[Trypsin and alpha-amylase activities in the pancreas of guinea pigs. III. Effect of homogenates of invasive ova of Ascaris suum and organic acids C2-C6 on the activity of enzymes in pancreatic extracts].

作者信息

Zółtowska K

机构信息

Zakład Cytologii i Genetyki, Instytut Biologii WSP.

出版信息

Wiad Parazytol. 1990;36(1-3):9-14.

PMID:2256339
Abstract

The studies were carried out on pancreas extracts from guinea pigs free of parasite invasion. The activity of trypsin was determined according to the method of Anson, and amylase of Fennel method. The measurements of activity were done at the presence of homogenized invasive eggs of Ascaris suum, and the mixture of volatile organic acids C2-C6 (at concentrations of 30.3 and 0.3 mM/l). In samples with homogenized invasive eggs of A. suum the amylase activity remained unchanged while the activity of trypsin was considerably higher (p less than 0.01). The introduction of the organic acids at conc. 30 mM/l into the samples was the reason of lower amylase activity. No influence of these acids on trypsin activity in pancreas extracts was observed.

摘要

这些研究是在没有寄生虫侵袭的豚鼠胰腺提取物上进行的。胰蛋白酶活性根据安森方法测定,淀粉酶活性根据芬内尔方法测定。活性测量是在存在猪蛔虫侵袭性虫卵匀浆以及挥发性有机酸C2 - C6混合物(浓度分别为30.3和0.3 mM/l)的情况下进行的。在含有猪蛔虫侵袭性虫卵匀浆的样本中,淀粉酶活性保持不变,而胰蛋白酶活性显著更高(p小于0.01)。向样本中引入浓度为30 mM/l的有机酸是淀粉酶活性降低的原因。未观察到这些酸对胰腺提取物中胰蛋白酶活性有影响。

相似文献

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