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[豚鼠胰腺中胰蛋白酶和α-淀粉酶活性。I. 1782年猪蛔虫感染剂量和感染持续时间的影响]

[Trypsin and alpha-amylase activity in the pancreas of guinea pigs. I. The influence of infective dose and duration of infection by Ascaris suum Goeze, 1782].

作者信息

Zółtowska K, Dziekońska-Rynko J, Jabłonowski Z

出版信息

Wiad Parazytol. 1989;35(6):565-70.

PMID:2633503
Abstract

The studies were carried out on guinea pig males weighting about 280 g. The animals were infected with 5000 or 20,000 invasive eggs of Ascaris suum. On the 3-rd, 7-th, 10-th and 14-th day of invasion the animals were dissected. Lungs were weighted, and the number of Ascaris larvae was estimated using Baermann method. The activity of alpha-amylase (Fennel's method) and trypsin (Anson's method) were examined in pancreas homogenate. The infection of guinea pigs with A. suum larvae resulted in a decrease of amylase and trypsin activity in pancreas. The activities were the lowest at the 3-rd day of invasion for amylase and at the 7-th day for trypsin. The results obtained for the animals infected 5 or 20,000 invasive eggs A. suum did not differ statically.

摘要

这些研究是在体重约280克的雄性豚鼠身上进行的。动物感染了5000或20000枚猪蛔虫侵袭性虫卵。在感染后的第3天、第7天、第10天和第14天对动物进行解剖。称取肺的重量,并使用贝尔曼法估计蛔虫幼虫的数量。在胰腺匀浆中检测α-淀粉酶(芬内尔法)和胰蛋白酶(安森法)的活性。豚鼠感染猪蛔虫幼虫导致胰腺中淀粉酶和胰蛋白酶活性降低。淀粉酶活性在感染后第3天最低,胰蛋白酶活性在第7天最低。感染5000或20000枚猪蛔虫侵袭性虫卵的动物所获得的结果在统计学上没有差异。

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