使用单克隆抗体12C3对上皮性卵巢癌中CD147蛋白表达的初步研究。
Pilot study of CD147 protein expression in epithelial ovarian cancer using monoclonal antibody 12C3.
作者信息
Ueda Kazu, Yamada Kyosuke, Kiyokawa Takako, Iida Yasushi, Nagata Chie, Hamada Tomomi, Saito Misato, Aoki Katsuhiko, Yanaihara Nozomu, Takakura Satoshi, Okamoto Aikou, Ochiai Kazunori, Ohkawa Kiyoshi, Tanaka Tadao
机构信息
Department of Obstetrics and Gynecology, The Jikei University School of Medicine, Tokyo, Japan.
出版信息
J Obstet Gynaecol Res. 2012 Sep;38(9):1211-9. doi: 10.1111/j.1447-0756.2012.01853.x. Epub 2012 May 8.
AIM
CD147 is a membrane glycoprotein that is expressed in various cancer cells and is involved in tumor invasion and metastasis by inducing stromal fibroblastic cells to produce matrix metalloproteinases. This study was carried out to evaluate the correlation between CD147 expression and various clinicopathologic parameters, including histological grade and prognosis in a small sample set of human ovarian cancer patients.
MATERIAL AND METHODS
Paraffin-embedded surgical tissue samples from 25 patients with ovarian serous and endometrioid adenocarcinoma were stained with anti-CD147 antibody (monoclonal antibody 12C3: MoAb 12C3) for immunohistochemical analysis.
RESULTS
CD147 protein was expressed in 84.0% (21 of 25 cases) of cancerous lesions, but not in normal lesions. CD147 expression by ovarian cancer cells was inversely correlated with overall survival. There was no correlation between CD147 expression and histological grade.
CONCLUSIONS
These results suggest that measurement of CD147 expression may enhance the understanding of the pathophysiology of epithelial ovarian cancer.
目的
CD147是一种膜糖蛋白，在多种癌细胞中表达，并通过诱导基质成纤维细胞产生基质金属蛋白酶参与肿瘤侵袭和转移。本研究旨在评估一小部分人类卵巢癌患者样本中CD147表达与各种临床病理参数（包括组织学分级和预后）之间的相关性。
材料与方法
对25例卵巢浆液性和子宫内膜样腺癌患者的石蜡包埋手术组织样本用抗CD147抗体（单克隆抗体12C3：MoAb 12C3）进行染色，以进行免疫组织化学分析。
结果
CD147蛋白在84.0%（25例中的21例）的癌性病变中表达，但在正常病变中不表达。卵巢癌细胞的CD147表达与总生存期呈负相关。CD147表达与组织学分级之间无相关性。
结论
这些结果表明，检测CD147表达可能有助于增强对上皮性卵巢癌病理生理学的理解。
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