Department of Biological Sciences, Cork Institute of Technology, Co. Cork, Ireland.
Gene. 2012 Aug 1;504(1):53-63. doi: 10.1016/j.gene.2012.04.085. Epub 2012 May 4.
Pediococcus damnosus (P. damnosus) bacteriophage (phage) clP1 is a novel virulent phage isolated from a municipal sewage sample collected in Southern Ireland. This phage infects the beer spoilage strain P. damnosus P82 which was isolated from German breweries. Sequencing of the phage has revealed a linear double stranded DNA genome of 38,013 base pairs (bp) with an overall GC content of 47.6%. Fifty seven open reading frames (ORFs) were identified of which 30 showed homology to previously sequenced proteins, and as a consequence 20 of these were assigned predicted functions. The majority of genes displayed homology with genes from the Lactobacillus plantarum phage phiJL-1. All genes were located on the same coding strand and in the same orientation. Morphological characterisation placed phage clP1 as a member of the Siphoviridae family with an isometric head (59 nm diameter) and non-contractile tail (length 175 nm; diameter 10nm. Interestingly, the phage clP1 genome was found to share very limited identity with other phage genome sequences in the database, and was hence considered unique. This was highlighted by the genome organisation which differed slightly to the consensus pattern of genomic organisation usually found in Siphoviridae phages. With the genetic machinery present for a lytic lifecycle and the absence of potential endotoxin factors, this phage may have applications in the biocontrol of beer spoilage bacteria. To our knowledge, this study represents the first reported P. damnosus phage genome sequence.
肠膜明串珠菌（Pediococcus damnosus）噬菌体（phage）clP1 是一种从爱尔兰南部采集的城市污水样本中分离出来的新型烈性噬菌体。这种噬菌体感染了从德国啤酒厂分离出来的啤酒污染菌株肠膜明串珠菌 P82。噬菌体的测序揭示了一个线性双链 DNA 基因组，大小为 38013 个碱基对（bp），总 GC 含量为 47.6%。鉴定出 57 个开放阅读框（ORFs），其中 30 个与已测序的蛋白质具有同源性，因此其中 20 个被赋予了预测的功能。大多数基因与植物乳杆菌噬菌体 phiJL-1 的基因显示出同源性。所有基因都位于同一编码链上，并具有相同的方向。形态特征将噬菌体 clP1 归类为肌尾噬菌体科的成员，具有等轴头（直径 59nm）和非收缩尾（长度 175nm；直径 10nm）。有趣的是，噬菌体 clP1 基因组与数据库中其他噬菌体基因组序列的相似度非常有限，因此被认为是独特的。这一点突出表现在基因组组织上，与肌尾噬菌体科噬菌体通常的基因组组织模式略有不同。由于存在裂解生命周期的遗传机制，并且缺乏潜在的内毒素因素，这种噬菌体可能在啤酒污染细菌的生物控制中有应用。据我们所知，这项研究代表了第一个报道的肠膜明串珠菌噬菌体基因组序列。
