Lin Po-Yen, Lee Shin-Shian, Chang Chia-Seng, Kao Fu-Jen
Institute of Biophotonics, National Yang-Ming University, Taipei, Taiwan.
Opt Express. 2012 May 7;20(10):11445-50. doi: 10.1364/OE.20.011445.
In this work, long working distance fluorescence lifetime imaging is realized with stimulated emission in combination with electronic time delay control. Spatial coherence, as a result of stimulated emission, supports unattenuated fluorescence detection at extended distance, using low NA optics. An electronic time delayed trigger provides an advantageous way in adjusting the pulse separation and probing the fluorescence lifetime in the nanosecond ranges. The fluorescence lifetime of selected fluorophores is accurately determined through the pump-probe configuration. The characteristics and applications in fluorescence lifetime measurement of stimulated emission are investigated and summarized succinctly here.
在这项工作中，通过受激发射结合电子时间延迟控制实现了长工作距离荧光寿命成像。受激发射产生的空间相干性支持使用低数值孔径光学器件在较长距离处进行无衰减荧光检测。电子时间延迟触发器为调整脉冲间隔和探测纳秒范围内的荧光寿命提供了一种有利方法。通过泵浦 - 探测配置准确测定所选荧光团的荧光寿命。本文简要研究并总结了受激发射在荧光寿命测量中的特性及应用。
