Kyrylkova Kateryna, Kyryachenko Sergiy, Leid Mark, Kioussi Chrissa
Department of Pharmaceutical Sciences, College of Pharmacy, Oregon State University, Corvallis, OR, USA.
Methods Mol Biol. 2012;887:41-7. doi: 10.1007/978-1-61779-860-3_5.
Terminal deoxynucleotidyl transferase (TdT) dUTP Nick-End Labeling (TUNEL) assay has been designed to detect apoptotic cells that undergo extensive DNA degradation during the late stages of apoptosis. The method is based on the ability of TdT to label blunt ends of double-stranded DNA breaks independent of a template. This chapter describes an assay for detection of apoptotic cells during mouse odontogenesis using a colorimetric TUNEL system.
末端脱氧核苷酸转移酶（TdT）介导的dUTP缺口末端标记（TUNEL）检测法旨在检测在凋亡后期经历广泛DNA降解的凋亡细胞。该方法基于TdT标记双链DNA断裂平端的能力，且无需模板。本章介绍了一种使用比色TUNEL系统检测小鼠牙齿发育过程中凋亡细胞的检测方法。
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