Yang Yang, Zhang Xin, Yang Jin, Wu Ziyan, Li Junpeng, Song Ruilong, Meng Chuang, Zhu Guoqiang
College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China.
Jiangsu Co-Innovation Center for Important Animal Infectious Diseases and Zoonoses, and Joint Laboratory of International Cooperation on Prevention and Control Technology of Important Animal Diseases and Zoonoses of Jiangsu, Yangzhou 225009, China.
Vet Sci. 2025 Mar 3;12(3):233. doi: 10.3390/vetsci12030233.
Quorum sensing (QS) is a process by which bacteria sense their population density and regulate behavior accordingly. QS not only regulates bacterial virulence but also directly influences host cells. Previous studies have shown that QS is strongly associated with piglet intestinal health, but the mechanism is not yet clear. For the first time, we have confirmed in a piglet animal model that OdDHL directly damages intestinal cells in weaned piglets, disrupting the intestinal barrier. We also provide a preliminary exploration of the underlying mechanism of these effects. TUNEL assays confirmed that damage to the piglet intestinal barrier coincided temporally and spatially with dysregulated apoptosis. Lipid rafts, key components of the cell membrane, are involved in many biological processes, including the activation of apoptosis-related proteins. Following the disruption of the lipid raft structure in IPEC-J2 cells, the apoptosis rate under OdDHL stimulation decreased by 50%. These data demonstrate that lipid rafts mediate the attachment of OdDHL to porcine intestinal cells; then, OdDHL induces apoptosis in porcine intestinal cells through the mitochondrial and death receptor pathways, thereby compromising the integrity of the porcine intestinal barrier. This study provides foundational insights into the role of QS in piglet intestinal diseases.
群体感应（QS）是细菌感知其群体密度并据此调节行为的过程。群体感应不仅调节细菌的毒力，还直接影响宿主细胞。先前的研究表明，群体感应与仔猪肠道健康密切相关，但其机制尚不清楚。我们首次在仔猪动物模型中证实，3-氧代十二烷酸（OdDHL）直接损害断奶仔猪的肠道细胞，破坏肠道屏障。我们还对这些影响的潜在机制进行了初步探索。TUNEL检测证实，仔猪肠道屏障的损伤在时间和空间上与凋亡失调一致。脂筏是细胞膜的关键组成部分，参与许多生物学过程，包括凋亡相关蛋白的激活。在IPEC-J2细胞中脂筏结构被破坏后，OdDHL刺激下的凋亡率降低了50%。这些数据表明，脂筏介导了OdDHL与猪肠道细胞的附着；然后，OdDHL通过线粒体和死亡受体途径诱导猪肠道细胞凋亡，从而损害猪肠道屏障的完整性。本研究为群体感应在仔猪肠道疾病中的作用提供了基础见解。
