Otsu Keishi, Fujiwara Naoki, Harada Hidemitsu
Division of Developmental Biology & Regenerative Medicine, Department of Anatomy, Iwate Medical University, Iwate, Japan.
Methods Mol Biol. 2012;887:59-67. doi: 10.1007/978-1-61779-860-3_7.
The study of organogenesis allows investigation of a variety of basic biological processes in the context of the intact organ. The ability to analyze teeth ex vivo during development has emerged as a powerful tool to understand how teeth are constructed and the signaling pathways that regulate these developmental processes. Here, we describe in detail our protocols for organ culture and kidney-capsule grafting of mouse tooth germs. These techniques allow us to reproduce the developmental process of tooth germs and estimate the effect of specific genes ex vivo, as well as are a tool for studies on the mechanisms of normal and abnormal tooth morphogenesis. They may also be applied to studies on other aspects of developmental biology and regenerative medicine.
器官发生的研究能够在完整器官的背景下探究各种基本生物学过程。在牙齿发育过程中对其进行离体分析的能力，已成为理解牙齿构建方式以及调控这些发育过程的信号通路的有力工具。在此，我们详细描述了小鼠牙胚的器官培养和肾包膜移植方案。这些技术使我们能够在离体条件下重现牙胚的发育过程并评估特定基因的作用，同时也是研究正常和异常牙齿形态发生机制的工具。它们还可应用于发育生物学和再生医学的其他方面的研究。
