Departamento de Biología Celular y Fisiología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Ciudad Universitaria, Apartado Postal 70228, Ciudad de México, DF 04510, Mexico.
Stem Cells Int. 2012;2012:675805. doi: 10.1155/2012/675805. Epub 2012 Feb 27.
We identify the presence of progenitor cells during retinal development in the dog, as this species represents a natural model for studying several breed-specific degenerative retinal disorders. Antibodies to detected progenitor cells (Pax6, C-kit, and nestin) and ganglion cells (BDNF, Brn3a, and Thy1) were used in combination with H3 for the purpose of identifying proliferating cells. Pax6, nestin, C-kit, and H3 were localized mainly in the neuroblastic layer of the retina during the embryonic stage. During the fetal stage, proteins were expressed in the inner neuroblastic layer (INL) as well as in the outer neuroblastic layer; BDNF, Thy1, and Brn3a were also expressed in the INL. During the neonatal stage only C-kit was not expressed. Proliferating cells were present in both undifferentiated and differentiated retina. These results suggest that, during canine retinogenesis, progenitor cells are distributed along the retina and some of these cells remain as progenitor cells of the ganglion cells during the first postnatal days.
我们在犬视网膜发育过程中鉴定出祖细胞的存在，因为该物种是研究几种品种特异性退行性视网膜疾病的天然模型。针对祖细胞（Pax6、C-kit 和巢蛋白）和神经节细胞（BDNF、Brn3a 和 Thy1）检测到的抗体与 H3 联合使用，用于鉴定增殖细胞。在胚胎期，Pax6、巢蛋白、C-kit 和 H3 主要定位于视网膜神经母细胞层。在胎儿期，这些蛋白在内神经母细胞层（INL）和外神经母细胞层中表达；BDNF、Thy1 和 Brn3a 也在 INL 中表达。在新生儿期，只有 C-kit 没有表达。增殖细胞存在于未分化和分化的视网膜中。这些结果表明，在犬视网膜发生过程中，祖细胞沿视网膜分布，其中一些细胞在出生后的头几天仍作为神经节细胞的祖细胞存在。
