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单链诱导RecA蛋白解开双链DNA以进行同源配对。

Single strands induce recA protein to unwind duplex DNA for homologous pairing.

作者信息

Cunningham R P, Shibata T, DasGupta C, Radding C M

出版信息

Nature. 1979 Sep 20;281(5728):191-5. doi: 10.1038/281191a0.

DOI:10.1038/281191a0
PMID:225671
Abstract

Single-stranded DNA, whether homologous or not, stimulates purified Escherichia coli recA protein to unwind duplex DNA. This helps to explain how recA promotes a search for homology in genetic recombination. As oligodeoxynucleotide also stimulate unwinding, a common mechanism may relate the function of recA protein in recombination to other functions (SOS) induced by oligonucleotides.

摘要

单链DNA，无论是否同源，都能刺激纯化的大肠杆菌recA蛋白解开双链DNA。这有助于解释recA如何在基因重组中促进同源性搜索。由于寡脱氧核苷酸也能刺激解旋，一种共同的机制可能将recA蛋白在重组中的功能与寡核苷酸诱导的其他功能（SOS）联系起来。

相似文献

1
Single strands induce recA protein to unwind duplex DNA for homologous pairing.单链诱导RecA蛋白解开双链DNA以进行同源配对。
Nature. 1979 Sep 20;281(5728):191-5. doi: 10.1038/281191a0.
2
Homologous pairing and topological linkage of DNA molecules by combined action of E. coli RecA protein and topoisomerase I.大肠杆菌RecA蛋白与拓扑异构酶I共同作用实现DNA分子的同源配对和拓扑连接
Cell. 1981 Apr;24(1):213-23. doi: 10.1016/0092-8674(81)90517-1.
3
Unwinding of heterologous DNA by RecA protein during the search for homologous sequences.RecA蛋白在寻找同源序列过程中对异源DNA的解旋。
J Mol Biol. 1992 Jul 5;226(1):127-39. doi: 10.1016/0022-2836(92)90129-8.
4
Homologous pairing between nucleosome cores on a linear duplex DNA and nucleoprotein filaments of RecA protein-single stranded DNA.线性双链DNA上的核小体核心与RecA蛋白-单链DNA的核蛋白细丝之间的同源配对。
Biochimie. 1991 Feb-Mar;73(2-3):187-90. doi: 10.1016/0300-9084(91)90201-b.
5
Unwinding associated with synapsis of DNA molecules by recA protein.RecA蛋白介导的DNA分子联会相关的解旋过程。
Proc Natl Acad Sci U S A. 1983 Mar;80(5):1256-60. doi: 10.1073/pnas.80.5.1256.
6
ATP-dependent unwinding of the double helix and extensive supercoiling by Escherichia coli recA protein in the presence of topoisomerase.在拓扑异构酶存在的情况下，大肠杆菌recA蛋白对双螺旋进行ATP依赖的解旋和广泛的超螺旋化。
J Biol Chem. 1983 Oct 25;258(20):12394-404.
7
RecA protein promoted homologous pairing in vitro. Pairing between linear duplex DNA bound to HU Protein (nucleosome cores) and nucleoprotein filaments of recA protein-single-stranded DNA.RecA蛋白在体外促进同源配对。与HU蛋白（核小体核心）结合的线性双链DNA与recA蛋白-单链DNA核蛋白细丝之间的配对。
J Biol Chem. 1989 Oct 15;264(29):17395-400.
8
RecBCD-dependent joint molecule formation promoted by the Escherichia coli RecA and SSB proteins.由大肠杆菌RecA和SSB蛋白促进的RecBCD依赖性联合分子形成。
Proc Natl Acad Sci U S A. 1991 Apr 15;88(8):3367-71. doi: 10.1073/pnas.88.8.3367.
9
ATP-dependent unwinding of double helix in closed circular DNA by recA protein of E. coli.
Nature. 1982 Sep 2;299(5878):86-9. doi: 10.1038/299086a0.
10
Homologous DNA pairing promoted by a 20-amino acid peptide derived from RecA.由源自RecA的一种20个氨基酸的肽所促进的同源DNA配对。
Science. 1996 May 10;272(5263):868-72. doi: 10.1126/science.272.5263.868.

引用本文的文献

1
Homology recognition without double-stranded DNA-strand separation in D-loop formation by RecA.RecA 形成 D 环过程中无需双链 DNA 链分离的同源识别。
Nucleic Acids Res. 2024 Mar 21;52(5):2565-2577. doi: 10.1093/nar/gkad1260.
2
The intrinsic ability of double-stranded DNA to carry out D-loop and R-loop formation.双链DNA进行D环和R环形成的内在能力。
Comput Struct Biotechnol J. 2020 Nov 4;18:3350-3360. doi: 10.1016/j.csbj.2020.10.025. eCollection 2020.
3
DNA Sequence Alignment during Homologous Recombination.同源重组过程中的DNA序列比对
J Biol Chem. 2016 May 27;291(22):11572-80. doi: 10.1074/jbc.R116.724807. Epub 2016 Apr 15.
4
RecBCD is required to complete chromosomal replication: Implications for double-strand break frequencies and repair mechanisms.RecBCD蛋白是完成染色体复制所必需的：对双链断裂频率和修复机制的影响。
DNA Repair (Amst). 2015 Aug;32:86-95. doi: 10.1016/j.dnarep.2015.04.018. Epub 2015 May 2.
5
A non-canonical DNA structure enables homologous recombination in various genetic systems.一种非经典DNA结构能够在多种遗传系统中实现同源重组。
J Biol Chem. 2009 Oct 30;284(44):30230-9. doi: 10.1074/jbc.M109.043810. Epub 2009 Sep 3.
6
Fluorescent detection and isolation of DNA variants using stabilized RecA-coated oligonucleotides.使用稳定的RecA包被寡核苷酸对DNA变体进行荧光检测和分离。
Genome Res. 2004 Jan;14(1):116-25. doi: 10.1101/gr.1386204. Epub 2003 Dec 12.
7
Conjugational hyperrecombination achieved by derepressing the LexA regulon, altering the properties of RecA protein and inactivating mismatch repair in Escherichia coli K-12.通过解除LexA调控子的抑制、改变RecA蛋白的特性以及使大肠杆菌K-12中的错配修复失活来实现接合超重组。
Genetics. 2003 Apr;163(4):1243-54. doi: 10.1093/genetics/163.4.1243.
8
The architecture of the human Rad54-DNA complex provides evidence for protein translocation along DNA.人类Rad54-DNA复合物的结构为蛋白质沿DNA的转运提供了证据。
Proc Natl Acad Sci U S A. 2001 Jul 17;98(15):8454-60. doi: 10.1073/pnas.151056798.
9
Recombinational repair of DNA damage in Escherichia coli and bacteriophage lambda.大肠杆菌和噬菌体λ中DNA损伤的重组修复
Microbiol Mol Biol Rev. 1999 Dec;63(4):751-813, table of contents. doi: 10.1128/MMBR.63.4.751-813.1999.
10
A novel property of the RecA nucleoprotein filament: activation of double- stranded DNA for strand exchange in trans.RecA核蛋白丝的一种新特性：双链DNA的反式链交换激活。
Genes Dev. 1999 Aug 1;13(15):2005-16. doi: 10.1101/gad.13.15.2005.