Beck L S, Chen T L, Mikalauski P, Ammann A J
Research and Development, Genetech Inc., South San Francisco, California 94080.
Growth Factors. 1990;3(4):267-75. doi: 10.3109/08977199009003669.
A new animal model to study secondary intention wound healing and the effects of topically applied rhTGF-beta 1 was developed. A time course study was performed of full thickness 6 mm punch wounds placed on the backs of anesthetized pigs and treated once with either 3% methylcellulose or rhTGF-beta 1 in 3% methylcellulose or left untreated. Wounds receiving rhTGF-beta 1 had enhanced tensile strength at days 4 and 7 compared to controls. Studies of the response on days 4 and 7 to graded doses of rhTGF-beta 1 showed that a dose of 250 or 2500 ng rhTGF-beta 1 gave a similar enhanced wound strength, while 25 ng rhTGF-beta 1 had no effect. Blood flow to treated granulating wounds as measured by 141Ce microspheres indicate an increase in flow in wounds treated with 250, 500 or 2500 ng rhTGF-beta 1 compared to controls. These results indicate a possible use for rhTGF-beta 1 in enhancing wound healing clinically.
开发了一种用于研究二期愈合伤口以及局部应用重组人转化生长因子β1（rhTGF-β1）效果的新动物模型。对麻醉猪背部的6毫米全层打孔伤口进行了一项时间进程研究，伤口分别用3%甲基纤维素、3%甲基纤维素中含rhTGF-β1处理一次，或不做处理。与对照组相比，接受rhTGF-β1处理的伤口在第4天和第7天的抗张强度增强。对第4天和第7天对不同剂量rhTGF-β1的反应研究表明，250或2500纳克rhTGF-β1剂量可产生类似的增强伤口强度效果，而25纳克rhTGF-β1则无效果。通过141铈微球测量，处理后的肉芽创面血流量显示，与对照组相比，用250、500或2500纳克rhTGF-β1处理的伤口血流量增加。这些结果表明rhTGF-β1在临床上增强伤口愈合方面可能具有应用价值。
