Department of Structural and Chemical Biology, Mt. Sinai School of Medicine, New York, NY 10029, United States.
Curr Opin Struct Biol. 2012 Aug;22(4):520-8. doi: 10.1016/j.sbi.2012.04.003. Epub 2012 May 8.
During the past year, electron crystallography of membrane proteins has provided structural insights into the mechanism of several different transporters and into their interactions with lipid molecules within the bilayer. From a technical perspective there have been important advances in high-throughput screening of crystallization trials and in automated imaging of membrane crystals with the electron microscope. There have also been key developments in software, and in molecular replacement and phase extension methods designed to facilitate the process of structure determination.
在过去的一年中,膜蛋白的电子晶体学为几种不同转运蛋白的机制以及它们与双层脂分子的相互作用提供了结构见解。从技术角度来看,在高通量筛选结晶试验和使用电子显微镜自动成像膜晶体方面取得了重要进展。在软件以及旨在促进结构确定过程的分子置换和相位扩展方法方面也取得了关键进展。