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微生物燃料电池阳极细菌群落的趋同演化。

Convergent development of anodic bacterial communities in microbial fuel cells.

机构信息

Department of Civil and Environmental Engineering, The Pennsylvania State University, University Park, PA 16802, USA.

出版信息

ISME J. 2012 Nov;6(11):2002-13. doi: 10.1038/ismej.2012.42. Epub 2012 May 10.

Abstract

Microbial fuel cells (MFCs) are often inoculated from a single wastewater source. The extent that the inoculum affects community development or power production is unknown. The stable anodic microbial communities in MFCs were examined using three inocula: a wastewater treatment plant sample known to produce consistent power densities, a second wastewater treatment plant sample, and an anaerobic bog sediment. The bog-inoculated MFCs initially produced higher power densities than the wastewater-inoculated MFCs, but after 20 cycles all MFCs on average converged to similar voltages (470±20 mV) and maximum power densities (590±170 mW m(-2)). The power output from replicate bog-inoculated MFCs was not significantly different, but one wastewater-inoculated MFC (UAJA3 (UAJA, University Area Joint Authority Wastewater Treatment Plant)) produced substantially less power. Denaturing gradient gel electrophoresis profiling showed a stable exoelectrogenic biofilm community in all samples after 11 cycles. After 16 cycles the predominance of Geobacter spp. in anode communities was identified using 16S rRNA gene clone libraries (58±10%), fluorescent in-situ hybridization (FISH) (63±6%) and pyrosequencing (81±4%). While the clone library analysis for the underperforming UAJA3 had a significantly lower percentage of Geobacter spp. sequences (36%), suggesting that a predominance of this microbe was needed for convergent power densities, the lower percentage of this species was not verified by FISH or pyrosequencing analyses. These results show that the predominance of Geobacter spp. in acetate-fed systems was consistent with good MFC performance and independent of the inoculum source.

摘要

微生物燃料电池(MFC)通常接种自单一废水来源。接种物对群落发展或产电的影响程度尚不清楚。本研究采用三种接种物(已知能稳定产生功率密度的污水处理厂样品、另一个污水处理厂样品和厌氧沼泽沉积物)来研究 MFC 稳定的阳极微生物群落。沼泽接种的 MFC 最初产生的功率密度高于废水接种的 MFC,但经过 20 个周期后,所有 MFC 的平均电压(470±20mV)和最大功率密度(590±170mW/m²)都趋同。沼泽接种的重复 MFC 的功率输出没有显著差异,但一个废水接种的 MFC(UAJA3(UAJA,大学区联合管理局污水处理厂))的产电量明显较少。变性梯度凝胶电泳分析显示,在经过 11 个周期后,所有样品中均存在稳定的异化电子生物膜群落。经过 16 个周期,使用 16S rRNA 基因克隆文库(58±10%)、荧光原位杂交(FISH)(63±6%)和焦磷酸测序(81±4%)确定了阳极群落中 Geobacter spp. 的优势地位。虽然表现不佳的 UAJA3 的克隆文库分析中 Geobacter spp.序列的比例明显较低(36%),表明该微生物的优势对于收敛的功率密度是必要的,但 FISH 或焦磷酸测序分析并未证实该物种的比例较低。这些结果表明,在乙酸盐喂养系统中 Geobacter spp.的优势与良好的 MFC 性能一致,且与接种物来源无关。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/716f/3475369/2440c3b622a6/ismej201242f1.jpg

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