Department of Earth Sciences, University of Southern California, Los Angeles, California 90089, United States.
Environ Sci Technol. 2011 Feb 1;45(3):1139-46. doi: 10.1021/es102645v. Epub 2010 Dec 21.
Electricity production by bacterial communities enriched from wastewater sludge with lactate, succinate, N-acetyl-D-glucosamine (NAG), acetate, formate, and uridine were monitored in dual-chamber microbial fuel cells (MFCs). Stable electricity production was observed after 300 h for communities enriched from lactate, acetate, and formate, while communities enriched with succinate, NAG, and uridine stabilized only after 700 h. The average peak current densities and maximum power densities generated from bacterial consortia were significantly higher than those generated from pure cultures of Shewanella oneidensis MR-1. Microbial assemblages were analyzed by DGGE, and planktonic and anode-attached bacterial communities varied as a function of electron donors: Firmicutes, β-Proteobacteria, and Bacteroidetes dominated the planktonic bacterial communities while anode-attached communities consisted mainly of δ-Proteobacteria, β-Proteobacteria, and Firmicutes. Similar bacterial populations were enriched in MFCs fed with lactate, NAG, and uridine and with succinate, acetate, and formate. Cross-feeding experiments with different fuels indicated that enriched microbial consortia were able to utilize a variety of fuel sources and displayed considerable stability, efficiency, and robustness of power generation in comparison to pure cultures. In addition, characterizations of cultivated Shewanella strains suggested that DGGE analysis likely missed active members of exoelectrogenic populations.
采用双室微生物燃料电池（MFC），对从废水污泥中富集的、以乳酸盐、琥珀酸盐、N-乙酰-D-葡萄糖胺（NAG）、乙酸盐、甲酸盐和尿嘧啶为电子供体的细菌群落进行了电能产生监测。从乳酸盐、乙酸盐和甲酸盐中富集的群落经过 300 h 后即可实现稳定的电能产生，而从琥珀酸盐、NAG 和尿嘧啶中富集的群落则需要 700 h 后才能稳定。与 Shewanella oneidensis MR-1 的纯培养物相比，细菌群落产生的平均峰值电流密度和最大功率密度要高得多。通过 DGGE 对微生物组合进行了分析，浮游和阳极附着细菌群落随电子供体的变化而变化：厚壁菌门、β-变形菌门和拟杆菌门是浮游细菌群落的优势菌群，而阳极附着群落主要由δ-变形菌门、β-变形菌门和厚壁菌门组成。在以乳酸盐、NAG 和尿嘧啶以及琥珀酸盐、乙酸盐和甲酸盐为电子供体的 MFC 中，富集了相似的细菌种群。不同燃料的交叉喂养实验表明，富集的微生物群落能够利用多种燃料源，与纯培养物相比，其发电具有相当的稳定性、效率和鲁棒性。此外，对培养的 Shewanella 菌株的特性进行了表征，表明 DGGE 分析可能错过了产电种群的活跃成员。
