一种测定红细胞中γ-谷氨酰环化转移酶活性的简单方法。
A simple method to determine gammaglutamyl-cyclotransferase activity in erythrocytes.
作者信息
Board P G, Smith J E, Moore K
出版信息
J Lab Clin Med. 1978 Jan;91(1):127-31.
PMID:22573
Abstract
A new method for the determination of erythrocyte gammaGCT is described. The method follows the release of alanine from gammaglutamyl alanine by a series of linked reactions that result in the oxidation of NADH to NAD. The pH optimum for human erythrocyte gammaGCT was found to be 9.0, and the Km for gammaglutamyl alanine was found to be 2.3 X 10(-3) M. Human erythrocytes had the highest activity of all the species studied (human, rabbit, dog, sheep, cattle, chicken).
摘要
本文描述了一种测定红细胞γ - 谷氨酰转肽酶（gammaGCT）的新方法。该方法通过一系列连锁反应使γ - 谷氨酰丙氨酸释放出丙氨酸，这些反应导致NADH氧化为NAD。发现人红细胞γ - 谷氨酰转肽酶的最适pH为9.0，γ - 谷氨酰丙氨酸的米氏常数（Km）为2.3×10⁻³ M。在所研究的所有物种（人、兔、狗、羊、牛、鸡）中，人红细胞的活性最高。
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