用于微量测定胶原酶活性的胶原膜。

A collagen film for microdetermination of collagenase activity.

作者信息

Giacomello A, Salerno C, Ferrari M, Giartosio A, Fasella P

出版信息

Physiol Chem Phys. 1979;11(2):169-73.

Abstract

A simple, rapid, sensitive, and specific film assay for collagenase activity employing a glass-supported, reconstituted collagen gel is described. Digestion of the collagen film results in sharply defined zones of lysis detectable by staining with Coomassie blue. The assay is semiquantitative and suitable for micro enzyme determination in biological fluids.

摘要

描述了一种使用玻璃支撑的重组胶原凝胶对胶原酶活性进行简单、快速、灵敏且特异的薄膜测定法。胶原薄膜的消化会产生可通过考马斯亮蓝染色检测到的界限清晰的溶解区域。该测定法是半定量的，适用于生物流体中的微量酶测定。