超声提取蛹虫草菌丝体多糖的理化特性。
Physicochemical characteristics of ultrasonic extracted polysaccharides from cordyceps cephalosporium mycelia.
机构信息
National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, PR China.
出版信息
Int J Biol Macromol. 2012 Jul-Aug;51(1-2):64-9. doi: 10.1016/j.ijbiomac.2012.04.029. Epub 2012 May 7.
Ultrasonic extraction (USE) of polysaccharide from cordyceps cephalosporium mycelia was introduced and compared with hot water extraction. Crude polysaccharides were investigated for morphological and thermodynamic properties. Two major fractions (USEP40-1, USEP70-1) were purified and investigated for IR spectra, molecular weights, monosaccharide compositions and antioxidant activities. The results suggested that USE gained higher extraction efficiency due to its intense extraction process. USE caused a more compact arrangement in crude polysaccharides. IR spectra of USEP40-1 and USEP70-1 were different at around 1746 cm(-1). The molecular weights of USEP40-1 and USEP70-1 were 61.4 kDa and 25.1 kDa. USEP40-1 and USEP70-1 were composed of D-mannose, D-glucose, D-galactose, L-rhamnose, and L-arabinose with molar ratio of 11.52:5.54:8.75:2.45:2.59 and 11.50:6.74:5.75:4.46:2.39, respectively. Different ABTS and hydroxyl radical scavenging activities were observed in USEP40-1 and USEP70-1.
超声提取（USE）法从蛹虫草菌丝体中提取多糖，并与热水提取法进行了比较。对粗多糖进行了形态和热力学性质的研究。对两种主要的多糖级分（USEP40-1、USEP70-1）进行了纯化，并对其红外光谱、分子量、单糖组成和抗氧化活性进行了研究。结果表明，由于超声提取过程强烈，USE 获得了更高的提取效率。USE 使粗多糖的排列更加紧密。USEP40-1 和 USEP70-1 的红外光谱在 1746cm(-1)左右不同。USEP40-1 和 USEP70-1 的分子量分别为 61.4 kDa 和 25.1 kDa。USEP40-1 和 USEP70-1 由 D-甘露糖、D-葡萄糖、D-半乳糖、L-鼠李糖和 L-阿拉伯糖组成，摩尔比分别为 11.52:5.54:8.75:2.45:2.59 和 11.50:6.74:5.75:4.46:2.39。在 USEP40-1 和 USEP70-1 中观察到不同的 ABTS 和羟基自由基清除活性。
Zotero 插件