School of Pharmacy, Jiangsu University, Zhenjiang, Jiangsu, China.
Int J Biol Macromol. 2011 Jun 1;48(5):798-803. doi: 10.1016/j.ijbiomac.2011.03.013. Epub 2011 Mar 30.
Grifola frondosa has been widely consumed in China and other Asian countries. Recent studies on G. frondosa have focused on the activities of polysaccharides extracted by water, and the activities of polysaccharides extracted by enzymolysis have not been studied. In this work, the relationship between the physical properties and antioxidant activity of polysaccharides extracted from G. frondosa by boiling water and enzymolysis was studied. Five polysaccharide extracts from the fruit body of G. frondosa were prepared by different extracting methods including boiling water, single enzyme enzymolysis with three different single enzymes (cellulose, pectinase, and pancreatin), and combined enzyme enzymolysis (cellulose:pectinase:pancreatin; 2:2:1). Characteristics such as the viscosity, Mw, polysaccharide content, protein content, infrared spectra, and antioxidant activities of the extracts were evaluated. The highest antioxidant activity was exhibited by the extracts prepared by combined enzyme extraction. The correlation analysis between antioxidant activity and polysaccharide content, protein content, Mw or viscosity indicated that the Mw had a more important role in antioxidant activity. Overall, the results indicate that the combined enzyme polysaccharide extracts can be developed as a new potential natural antioxidant.
灰树花在我国和其他亚洲国家被广泛食用。最近对灰树花的研究集中在水提多糖的活性上，而对酶解提取多糖的活性则没有研究。在这项工作中，研究了热水提取和酶解提取的灰树花多糖的物理性质与抗氧化活性之间的关系。采用不同的提取方法，包括热水提取、三种不同的单一酶（纤维素酶、果胶酶和胰蛋白酶）单一酶解和复合酶解（纤维素：果胶酶：胰蛋白酶；2：2：1），从灰树花子实体中制备了五种多糖提取物。评价了提取物的粘度、Mw、多糖含量、蛋白质含量、红外光谱和抗氧化活性等特性。通过复合酶提取制备的提取物表现出最高的抗氧化活性。抗氧化活性与多糖含量、蛋白质含量、Mw 或粘度之间的相关性分析表明，Mw 在抗氧化活性中起着更重要的作用。总的来说，研究结果表明，复合酶多糖提取物可以作为一种新的潜在天然抗氧化剂进行开发。
